干扰素诱导蛋白-10在肝源性糖尿病中的表达及意义  

Expression of interferonγ-inducible protein 10 in hepatic diabetes

作　　者：刘晓燕[1] 吕勇[1] 王艳丽[1] 朱金照[1] 崔永良[1] 熊伟[1] 韩荔芬[1] 

机构地区：[1]福建医科大学孟超肝胆医院消化科,福州350025

出　　处：《福建医药杂志》2017年第1期75-78,F0004,共5页Fujian Medical Journal

基　　金：福州市科技计划项目(2014-S-139-8)

摘　　要：目的检测干扰素诱导蛋白-10(interferon-induced protein 10,IP-10)在肝组织及血清中的表达浓度,探讨其与肝源性糖尿病的关系。方法应用ELISA、Real-time PCR方法检测60例肝源性糖尿病组患者和60例普通肝病组患者的血清、肝组织中IP-10表达水平,免疫组化方法检测两组患者肝组织中IP-10表达阳性率。结果在肝源性糖尿病组患者血清中IP-10的浓度为(239.54+28.60)pg/mL,明显高于普通肝病组的(205.34+26.95)pg/mL(P<0.05);在肝源性糖尿病患者肝组织中lg IP-10与lg GAPDH比值为0.55+0.92,也明显高于普通肝病组的0.26+0.64。免疫组化法检测肝源性糖尿病组肝组织IP-10阳性率30%明显高于普通肝病患者11.7%(P<0.05)。结论 IP-10高表达可能通过炎症机制与肝源性糖尿病的发生有关系。Objective To detect the expression of interferonγ-inducible protein 10（IP-10）in serum and liver tissues,and to investigate whether IP-10 is involved in the inflammatory process of the hepatic diabetes.Methods A total of 120 patients were divided into the liver disease complicated with hepatic diabetes group and the liver disease without diabetes group on average.The levels of IP-10 in serum and liver tissues from two groups were measured by the method of ELISA,Real-time PCR and Streptavidin-peroxidase immunohistochemistry stain.Results The level of IP-10 in serum of patients with hepatic diabetes group was（239.54±28.60）pg/mL,which was higher than that without diabetes group（205.34±26.95）pg/mL,and the difference had statistically significant（P〈0.05）.The level of IP-10 in liver tissue of patients with hepatic diabetes group was（0.55±0.92）lgcDNA/lgGAPDH,which was higher than that without diabetes group（0.26±0.64）lgcDNA/lgGAPDH,and the difference had statistically significant（P〈0.05）.By Streptavidin-peroxidase immunohistochemistry stain method,IP-10 protein expression in liver tissues of patients with hepatic diabetes group was 30%,which was higher than that without diabetes group 11.7%,and the difference had statistically significant（P〈0.05）.Conclusion There is high level of IP-10 protein expression in serum and liver tissue of patients with hepatic diabetes,which may be correlated with the inflammatory process in hepatic diabetes.

关 键 词：干扰素诱导蛋白-10 肝源性糖尿病 炎症趋化因子 

分 类 号：R587.1[医药卫生—内分泌]