金银花肌动蛋白基因LjActin的克隆及在花发育过程中的表达分析  

Cloning of Actin Gene Lj Actin in Lonicera japonica and Its Expression at Different Stages of Flower Development

作　　者：亓希武[1] 徐道华[1] 于盱[1] 房海灵[1] 李维林[1] 梁呈元[1] QI Xi-wu XU Dao-hua YU Xu FANG Hai-ling LI Wei-lin LIANG Cheng-yuan(Institute of Botany, Jiangsu Provincial and Chinese Academy of Sciences, Nanjing 210014, China)

机构地区：[1]江苏省中国科学院植物研究所,江苏南京210014

出　　处：《江西农业学报》2017年第3期90-94,共5页Acta Agriculturae Jiangxi

基　　金：国家自然科学基金青年基金项目(31500249);江苏省"六大人才高峰"资助项目(2015-NY-032);江苏省中国科学院植物研究所青年基金项目(SQ201401)

摘　　要：本研究在药用植物金银花中克隆得到1条肌动蛋白(Actin)基因序列,命名为Lj Actin(Gen Bank登录号:KY114518)。序列分析表明其全长1536 bp,其中编码框1134 bp,编码377个氨基酸残基,理论分子量为41.7 k Da,理论等电点为5.31。序列比对结果表明金银花的Actin和其它植物的Actin在序列上高度保守。进化分析结果表明金银花的Actin与拟南芥的At ACT7和水稻的Os ACT2聚为一支。RT-PCR结果显示Lj Actin在金银花5个不同发育时期的花中表达稳定。In this study,we cloned an actin gene Lj Actin（ Gen Bank accession number： KY114518） from medicinal plant Lonicera japonica Thunb. The results of sequence analysis indicated that： the total length of Lj Actin was 1536 bp,and its ORF was1134 bp encoding 377 amino acids; this actin gene possessed the theoretical molecular weight of 41.7 k Da and the theoretical isoelectric point of 5.31. Multiple sequence alignment showed that the sequence of Lj Actin in L. japonica was highly conserved as that in other plants. Phylogenetic analysis showed that the Lj Actin in L. japonica was clustered into a clade with the At ACT7 in Arabidopsis thaliana and the Os ACT2 in rice（ Oryza sativa）. The result of RT-PCR indicated that Lj Actin could steadily express in the flowers of L. japonica at 5 different developmental stages.

关 键 词：金银花 肌动蛋白 基因 克隆 表达 

分 类 号：Q943.2[生物学—植物学]