测定CD38^+CD8^+T淋巴细胞在监测肾移植后巨细胞病毒感染中的作用  

Role of CD38^+CD8^+ T lymphocyte on monitoring cytomegalovirus infection after kidney transplantation

作　　者：费继光[1] 陈立中[1] 王长希[1] 郑克立[1] 吴培根[2] 孔庆喻[3] 

机构地区：[1]中山大学附属第一医院器官移植科,广州510080 [2]中山大学附属第一医院肾内科,广州510080 [3]中山大学附属第一医院肾病实验室,广州510080

出　　处：《中华肾脏病杂志》2002年第4期251-253,共3页Chinese Journal of Nephrology

摘　　要：目的 观察CD38+CD8+T淋巴细胞水平在肾移植后巨细胞病毒感染患者体内的变化,探讨其监测肾移植后巨细胞病毒活动性感染的可能性。方法 分别应用流式细胞术和免疫组织化学方法测定56例肾移植受者手术前后的CD38+CD8+T淋巴细胞水平和巨细胞病毒白细胞抗原,并将两者结果进行比较。结果 肾移植术前所有患者巨细胞病毒白细胞抗原均为阴性,其(CD38+CD8+)/CD8+的平均比值为0.11±0.05;肾移植后检测到有14例患者巨细胞病毒白细胞抗原阳性,出现阳性的时间为术后(32.7±16.6)d,(CD38+CD8+)/CD8+的比值在术后(29.6±8.4)d出现了有显著意义的升高,平均数值为0.43±0.21。这些患者接受静脉滴注丙氧鸟苷治疗后巨细胞病毒白细胞抗原转阴,(CD38+CD8+)/CD8+平均比值下降为0.16±0.09。治疗前后CD38+CD8+T淋巴细胞水平比较差异有显著性意义(P<0.05)。结论 CD38+CD8+T淋巴细胞水平的检测结合巨细胞病毒白细胞抗原检查有助临床监测肾移植后CMV活动性感染。Objective To examine the levels of CD38+CD8+ T lymphocyte in kidney transplant recipients with cytomegalovirus infection and investigate the possibility in monitoring the cytomegalovirus active infection after kidney transplantation. Methods Before and after kidney transplantation, CD38+ CD8+T lymphocyte and cytomegalovirus leucocyte antigen were measured respectively by flow cytometry and immunohistochemistry method in 56 transplant recipients. The data of CD38+CD8+ T lymphocyte and the cylomegalovirus leucocyte antigen were analyzed. Results Before kidney transplantation, cytomegalovirus leucocyte antigen was negative among all the patients, while the mean ratio of (CD38+CD8+)/CD8+ was 0. 11±0. 05. In 14 recipients whose cytomegalovirus leucocyte antigen was positive, the appearing time of the positive antigen was(32. 7±16. 6) days of post-transplantation, meanwhile, the mean ratio of (CD38+ CD8+)/CD8+ was 0. 43±0. 21 (29. 6±8. 4) days of post-transplantation, which was significantly higher than that of pre-transplantation. After the treatment with ganciclovir intravenously, the cytomegalovirus leucocyte antigen became negative and the mean ratio of (CD38+CD8+)/CD8+ decreased to 0. 16±0.09 which was significantly lower than that of pre-treatmmt ( P < 0. 05). Conclusion Measurement of CD38+ CD8+ T lymphocyte level combined with cytomegalovirus leucocyte antigen is useful in monitoring clinical cytomegalovirus active infection after kidney transplantation.

关 键 词：CD38^+ CD8^+ 肾移植 巨细胞病毒感染 T淋巴细胞 白细胞抗原 流式细胞术 免疫组织化学 

分 类 号：R699[医药卫生—泌尿科学]