四川猕猴桃软腐病防治初步研究  

Preliminary Study on Control Efficiency against Soft Rot Disease of Kiwifruit in Sichuan

作　　者：胡容平[1] 石军 林立金[3] 叶慧丽[1] 姚琳[1] 周游[3] 

机构地区：[1]四川省农业科学院植物保护研究所/农业部西南作物有害生物综合治理重点实验室,四川成都610066 [2]绵阳市农业科学研究院,四川绵阳621023 [3]四川农业大学果蔬研究所,四川成都611130

出　　处：《西南农业学报》2017年第2期366-370,共5页Southwest China Journal of Agricultural Sciences

基　　金：四川省应用基础项目"猕猴桃软腐病菌遗传多样性及致病力;抗性评价体系研究"(2013JY0115);四川省科技支撑项目"利用荧光定量PCR技术建立猕猴桃软腐病检测预测体系"(2015NZ0036);四川省创新能力提升工程项目"四川猕猴桃储藏期病害主要种类;发生特点及防治技术研究"(2014CXSF-018)

摘　　要：本试验以木霉菌(Trichoderma spp.)为生防筛选材料,针对猕猴桃软腐病(葡萄座腔菌Botryosphaeria dothidea),通过对峙培养、难挥发性代谢产物的拮抗试验以及5种杀菌剂对病原菌的毒力测定,为猕猴桃软腐病生物、化学防治提供参考的理论依据。结果表明,菌株T156-37对葡萄座腔菌的拮抗效果最好,抑制率达到66.11%。木霉菌株T163-8的难挥发性代谢产物对葡萄座腔菌的抑制作用最好,抑制率达到63.26%。25%咪鲜胺在室内生测试验效果显著,其次是30%苯醚甲环唑.丙环唑,EC50(μg/m L)分别是0.3825和10.2351。Trichoderma spp. , as the biocontrol selection material, was used to focus on the biocontrol of kiwifruit soft rot （ Botryosphaeria do- thidea） in this experiment, the confrontation culture of T. spp. and its antagonistic tests on non-volatile metabelites were conducted, and the virulence of five kinds of fungicides to pathogen were determined to provide a reference for the biochemical and chemical control of kiwifruit soft rot. The results showed that the strain T156-37 had the best antagonistic effects on B. dothidea, and the inhibition rate reached 66.11 %. The inhibition rate of non-volatile metabolites in Tr/choderma strain T163-8 was 63.26 %. The 25 % prochloraz in the indoor test re- suits were significant, followed by the 30 % difenoconazoleyl, propiconazole, which ECso （μg/mL） were 0.3825 and 10. 2351, respective- ly.

关 键 词：猕猴桃 软腐病 木霉 生物防治 毒力测定 

分 类 号：S436.634[农业科学—农业昆虫与害虫防治]