暖温带森林土壤酶活性对增温的响应及其环境解析  

Response of Soil Enzyme Activities to Soil Warming and Explanation of Environmental Factors in Warm-temperate Oak Forest

作　　者：王一[1] 刘彦春[1] 刘世荣[1] WANG Yi LIU Yan-chun LIU Shi-rong(Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Ecology and Environment, State Forestry Administration, Beijing 100091, China)

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所,国家林业局森林生态环境重点实验室,北京100091

出　　处：《林业科学研究》2017年第1期117-124,共8页Forest Research

基　　金：国家自然科学基金重大项目“植物对森林生态系统碳-氮-水耦合循环的作用机制”(项目编号:31290223);中国林业科学研究院基本科研业务费专项资金项目“海内外杰出学者讲习计划”(项目编号:CAFYBB2011007)

摘　　要：[目的]模拟气候变化背景下土壤酶活性季节变化规律和土壤酶活性对环境因子变异的响应,以深入理解气候变化背景下微生物因素在调控土壤碳循环过程中的作用。[方法]以暖温带锐齿栎林土壤为研究对象,采用红外辐射增温技术和96孔-微平板法测定了生长季和非生长季土壤水解酶和氧化酶活性,解析环境因子在调控土壤酶活性季节变异中的作用。[结果]显示:增温处理在生长季显著提高土壤温度1.91℃,导致土壤有机碳含量显著降低了12.15%,微生物量碳氮显著增加了40.30%和61.29%;增温处理在非生长季显著提高土壤温度2.24℃,但对微生物量碳氮影响并不显著。增温处理对土壤专一酶活性的影响存在季节差异,生长季除N-乙酰-葡糖苷酶外增温处理降低了土壤专一酶活性;非生长季除β-葡糖苷酶和蛋白酶外增温处理增加了土壤专一酶活性。环境因子可以解释土壤专一酶活性变异的82.5%,MBN和NH4-N极显著影响土壤专一酶活性,二者共可以解释变异的59.0%。[结论]MBN和NH4-N是影响土壤专一酶活性的主要环境因子,其他环境因子:土壤水热配比、微生物量碳、微生物量碳氮比、溶解性有机碳和硝态氮等是影响土壤专一酶活性在处理间和季节间变异的主要驱动因子。[Objective]To study the seasonal variation of soil enzyme activities and its response to environmental factors and to understand the effect of microbial factor in the regulation of soil carbon cycle in the context of climate change. [Method]The infrared radiation heater and the 96 micro-plate method were used to assay the activity of the hydrolytic and oxidative enzymes of the soil,and determine the role of environmental factors in addressing the seasonal variation in the activity of soil enzymes. [Result]During growing season,the warming treatment significantly increased the soil temperature by 1. 91℃,leading to a 12. 15% decrease of organic carbon content and significant increase of 40. 30% and 61. 29% of microbial biomass carbon（ MBC） and nitrogen（ MBN）. During nongrowing season,however,although the warming treatment increased the soil temperature by 2. 24℃,no significant effect was found on MBC or MBN. The effects of warming treatment on specific enzyme varied in different seasons.During growing season,the activities of specific enzyme in warming treatment were lower than those in control exceptfor NAG,while they were higher during non-growing season except for BG and LAP. [Conclusion]The environmental factors accounted for 82. 5% of the total variations of soil enzyme activities,while the activities of specific enzyme were significantly affected by NH4-N and MBN. The other environmental factors（ soil moisture,MBC,DOC,MBC / MBN and NO3-N） were the main reasons for the variations of specific enzyme activities in different treatments and seasons.

关 键 词：暖温带 锐齿栎林 土壤酶活性 增温 环境解析 

分 类 号：S714[农业科学—林学]