木犀草素体外对H_1N_1感染A549作用的研究  

Intervention Effect of Luteolin on A549 infected by H_1N_1 in Vitro

作　　者：邓东沅 顾立刚[1] 刘晓婷[1] 于卓男[1] 吴珺[1] 邱泽计[1] 王玥琦[2] 

机构地区：[1]北京中医药大学基础医学院中医药防治病毒性疾病实验室,北京100029 [2]北京中医药大学基础医学关键技术中心,北京100029

出　　处：《中医药导报》2017年第3期11-14,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金资助项目(No.81173371)

摘　　要：目的:观察木犀草素体外对感染甲型流感病毒(H_1N_1)的人肺癌细胞株(A549细胞株)的效果,探讨木犀草素在体外环境中对H_1N_1感染A549细胞的作用。方法:采用细胞病变效应(CPE)预估木犀草素抗病毒活性指数,计算出最大无毒浓度(TC0)、半数毒性浓度(TC50);检测不同时间和作用方式时木犀草素的吸光度,找出木犀草素抗病毒最佳作用方式,并计算出半数抑制浓度(IC50)以及治疗指数(TI)。结果:木犀草素的半数中毒浓度为39.81μg·m L^(-1),最大无毒浓度为25.00μg·m L^(-1);浓度在12.50~25.00μg·m L^(-1),木犀草素作用48~60 h,对H_1N_1所致的细胞病变作用有明显的抑制作用。病毒感染后2 h加入25.00μg·m L^(-1)浓度的木犀草素,抗病毒有效率最高为(87.00±1.71)%。计算该条件下木犀草素半数抑制浓度为11.282μg·m L^(-1),治疗指数为3.528。结论:木犀草素在一定条件下具有抗H_1N_1的作用。Objective： To observe the role of Luteolin after the infection of influenza virus （H1N1） in human lung cancer cell line （A549 cell line）. Methods： The maximum of non-toxic concentration （TCo） and the median toxic concentration （TC50） were detected by the cytopathogenic effect （CPE） of cells. Furthermore, The median inhibitory concentration （IC50） and treatment indexes （TI） in the best mode were calculated by Probit regression. Results： The median toxic concentration of Luteolin was 39.810μg·m L^-1, and maximum non-toxic concentration was 25 μg·m L^-1; A significant inhibitory effect was shown when added luteolin from 12.5 to 25.0 μg·m L^-1 after 48-60 h compared with the model without the drug. In addition, the best method against viral infection was adding Luteolin at 2 after cells infected and the best survival rate was （87.00±1.71） % with 25.0 μg·m L^-1 of Luteolin. Under this condition, IC50 was 11.282 μg·m L^-1, and TI was 3.528. Conclusion：Luteolin possesses the effect of anti-H1NI virus in vitro.

关 键 词：木犀草素 体外 A549 H1N1 

分 类 号：R285.5[医药卫生—中药学]