正畸牙移动中热休克蛋白70在牙周组织中的表达  

Expression of heat shock protein 70 in periodontium during orthodontic tooth movement

作　　者：聂晶[1] 姜涵[2] 李泽慧[2] 潘旭[1] 李春霞[1] Nie Jing Jiang Han Li Ze-hui Pan Xu Li Chun-xia(Department of Orthodontics Department of Periodontics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China)

机构地区：[1]新疆医科大学第一附属医院口腔正畸科,新疆维吾尔自治区乌鲁木齐市830054 [2]新疆医科大学第一附属医院牙周粘膜科,新疆维吾尔自治区乌鲁木齐市830054

出　　处：《中国组织工程研究》2017年第4期559-563,共5页Chinese Journal of Tissue Engineering Research

基　　金：新疆维吾尔自治区自然科学基金青年项目(2014211C080)~~

摘　　要：背景:学者们对正畸力作用下牙周组织的改建做了大量的研究,但对于在大鼠牙齿移动过程中热休克蛋白70在牙周组织中表达的研究尚未涉及。目的:观察在正畸牙移动过程中大鼠牙周组织热休克蛋白70的表达。方法:采用NiT i拉簧建立大鼠正畸牙移动模型,力值50 g。分别于加力后1,3,5,7,10,14 d检测正畸牙周组织热休克蛋白70的表达。结果与结论:(1)免疫荧光染色显示,正畸加力1 d,牙周组织中热休克蛋白70的表达量增强,至加力后5 d时表达量最大,之后逐渐降低,第14天时回落至初始水平;(2)结果说明,正畸力可以刺激牙周组织中热休克蛋白70的表达,热休克蛋白70可能参与了牙周组织的改建。BACKGROUND： Extensive research has been concentrated on the reconstruction of periodontium under orthodontic force. However, there are no reports regarding the expression of heat shock protein 70（HSP70） in rat periodontium during orthodontic tooth movement. OBJECTIVE： To investigate the expression of HSP70 in rat periodontium during orthodontic tooth movement. METHODS： Rat orthodontic tooth movement models were established using Ni Ti extension spring with the force of 50 g. The orthodontic periodontium was collected at 1, 3, 5, 7, 10 and 14 days after exerting force todetect the expression of HSP70. RESULTS AND CONCLUSION： According to immunofluorescence staining, the expression of HSP70 in the periodontal tissues was increased on day 1 after exerting force, and reached the highest level on day 5, then decreased to the initial level on day 14. These results suggest that the expression of HSP70 is evoked by orthodontic force, and it may play a role in periodontal remodeling.

关 键 词：组织工程 热休克蛋白质类 成骨细胞 组织构建 热休克蛋白 牙周组织 正畸 牙移动 大鼠 免疫荧光 组织改建 破骨细胞 新疆维吾尔自治区自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]