血管内皮细胞生长因子与碱性成纤维细胞生长因子联合应用对鼠牙周膜成纤维细胞增殖与碱性磷酸酶活性的影响  

Effects of vascular endothelial growth factor combined with basic fibroblast growth factor on periodontal ligament fibroblast proliferation and alkaline phosphatase activity in rats

作　　者：曹宇[1] 王莉莉 Cao Yu Wang Li-li(Department of Stomatology, the Sixth People＇s Hospital of Shenyang, Shenyang 110000, Liaoning Province, China Department of Prosthodontics, the Affiliated Stomatological Hospital of Jinzhou Medical University, Jinzhou 121004, Liaoning Province, China)

机构地区：[1]沈阳市第六人民医院口腔科,辽宁省沈阳市110000 [2]锦州医科大学附属口腔医院修复科,辽宁省锦州市121004

出　　处：《中国组织工程研究》2017年第4期580-585,共6页Chinese Journal of Tissue Engineering Research

基　　金：辽宁省科技厅自然科学基金优秀人才培育项目(2014022003);辽宁省大学生创新训练项目(201510160000045)~~

摘　　要：背景:碱性成纤维细胞生长因子可以提高成纤维细胞的增殖及胶原的沉积能力,血管内皮细胞生长因子可改善病变组织的血流灌注和新陈代谢水平,且两者在适当的浓度、时间作用下,均可增强碱性磷酸酶的活性。目的:观察血管内皮细胞生长因子与碱性成纤维细胞生长因子联合应用对鼠牙周膜成纤维细胞增殖与碱性磷酸酶活性的影响。方法:(1)体外培养大鼠牙周膜成纤维细胞并鉴定其胚胎来源,第4代细胞用于实验;(2)检测不同浓度血管内皮细胞生长因子、碱性成纤维细胞生长因子对大鼠牙周膜成纤维细胞增殖的影响,并从中确定显效浓度及最大效应浓度;(3)将细胞分为5组,分别为A组:对照组,为含体积分数2%胎牛血清的DMEM;B组:血管内皮细胞生长因子最大效应浓度组;C组:碱性成纤维细胞生长因子最大效应浓度组;D组:血管内皮细胞生长因子显效浓度与碱性成纤维细胞生长因子显效浓度联合应用;E组:血管内皮细胞生长因子最大效应浓度与碱性成纤维细胞生长因子最大效应浓度联合应用。于3,7,14 d后,测定5组细胞的碱性磷酸酶活性。结果与结论:(1)培养的大鼠牙周膜成纤维细胞生长状态良好,来源于中胚层;(2)随着血管内皮细胞生长因子、碱性成纤维细胞生长因子质量浓度的增加,大鼠牙周膜成纤维细胞增殖能力也随之提高(P<0.01)。血管内皮细胞生长因子的显效浓度为10μg/L,最大效应浓度为100μg/L;碱性成纤维细胞生长因子的显效浓度为0.1μg/L,最大效应浓度为10μg/L;(3)当血管内皮细胞生长因子、碱性成纤维细胞生长因子联合作用于大鼠牙周膜成纤维细胞时,实验各组A值均高于对照组。(4)在应用3 d和7 d时,但D组A值显著低于E组(P<0.05),14 d时2组差异无显著性意义。(5)结果说明,在一定作用时间内,最大效应浓度联合应用对碱性磷酸酶的活性能起到明显的协同作用,但超过BACKGROUND： Basic fibroblast growth factor（b FGF） can enhance fibroblast proliferation and collagen deposition, and vascular endothelial growth factor（VEGF） can improve blood perfusion and metabolic level of pathological tissues. Additionally, both of them can boost the alkaline phosphatase activity under given conditions. OBJECTIVE： To evaluate the effect of b FGF combined with VEGF on the periodontal ligament fibroblast proliferation and alkaline phosphatase activity in rats. METHODS： Rat periodontal ligament fibroblasts were cultured in vitro, its embryonic origin was identified and passage 4 cells were used for the following experiments. Effects of b FGF and VEGF with different concentrations on the rat periodontal ligament fibroblast proliferation were detected to determine the minimum and maximum effective concentrations. Cells were divided into five groups： group A（control group） with DMEM containing 2% fetal bovine serum; group B as maximum effective concentration of VEGF group; group C as maximum effective concentration of b FGF; group D as minimum effective concentration of b FGF combined with minimum effective concentration of VEGF group; group E as maximum effective concentration of b FGF combined with maximum effective concentration of VEGF group. At 3, 7 and 14 days, the alkaline phosphatase activity in each group was detected. RESULTS AND CONCLUSION： Rat periodontal ligament fibroblasts derived from the mesoderm grew well. Rat periodontal ligament fibroblast proliferation was increased with the VEGF and b FGF concentration increasing（P〈0.01）. The maximum and minimum effective concentrations of VEGF were 100 and 10 μg/L, and the maximum and minimum effective concentrations of b FGF were 10 and 0.1 μg/L. The absorbance values in the groups D and E were higher than those in the group A. The absorbance values of the group D were significantly lower than those of the group E at 3 and 7 days（P〈0.05）, but did not significantly differ at 14 days（P〉0.05）. To conclud

关 键 词：成纤维细胞生长因子2 血管内皮生长因子 牙周膜 碱性磷酸酶 组织构建 组织工程 碱性成纤维细胞生长因子 牙周膜成纤维细胞 体外培养 增殖 

分 类 号：R318[医药卫生—生物医学工程]