诱导多能干细胞的安全性及应用研究进展  

The safety and application of induced pluripotent stem cells

作　　者：田胜男[1] 王博[1] 李琦[1] 黄元华[1] 马燕琳[1] Tian Sheng-nan Wang Bo Li Qi Huang Yuan-hua Ma Yan-lin(First Affiliated Hospital of Hainan Medical University, Haikou 570102, Hainan Province, China)

机构地区：[1]海南医学院第一附属医院,海南省海口市570102

出　　处：《中国组织工程研究》2017年第5期815-820,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家国际科技合作专项(2014DFA30180);国家科技部973项目(2012CB966502);国家自然科学基金(81060175;30860103;81460034;81260032;81060016;31140021);海南省国际科技合作专项(GJXM20100004;GJXM201106;KJHZ2014-11);人力资源和社会保障部2011年度留学人员科技活动择优资助项目;教育部归国留学人员启动基金项目;海南医学院创新性实验项目(HYCX201304)~~

摘　　要：背景:诱导多能干细胞技术解决了伦理学和免疫排斥等矛盾,高效安全定向诱导多能干细胞技术成了当今研究的主流。目的:综述诱导多能干细胞的诱导安全性和应用进展。方法:应用计算机检索Pub Med及中国期刊全文数据库2006年1月至2016年3月有关诱导多能干细胞的文章,英文检索词为"induced pluripotent stem cell,reprogramming,clinical application,safety,transcription factor,disease model",中文检索词为"诱导多能干细胞,重编程,临床应用,遗传安全性,转录因子,疾病模型"。结果与结论:近年来诱导多能干细胞的研究备受科研界和医学界的关注,并努力克服了免疫和伦理问题,成功获得了诱导多能干细胞。但由于无法解决诱导多能干细胞技术中的安全性、效率和诱导多能干细胞细胞再分化机制等方面的问题,而局限于理论和实验室研究,迟迟无法应用于临床。如何安全高效的将诱导多能干细胞诱导为患者需要的类型并进行移植,如何建立良好的疾病模型与高通量药物筛选平台,涉及各方面的基础研究,面临着巨大的困难和挑战。BACKGROUND： Induced pluripotent stem cell technology have solved the contradiction between the ethics and immune rejection, and this high-efficient and safe technique is becoming the mainstream of today＇s research. OBJECTIVE： To comprehensively review the safety and application of induced pluripotent stem cells.METHODS： A computer-based online retrieval of Pub Med and CNKI was performed to search relevant papers published from January 2006 to April 2016, with the key words of ＂induced pluripotent stem cell, reprogramming, clinical application, safety, transcription factor, disease mode＂ in English and Chinese, respectively. RESULTS AND CONCLUSION： In recent years, research on induced pluripotent stem cells has attracted much attention from the scientific community and the medical community, and this technique has successfully gained induced pluripotent stem cells and overcome the problems of immunity and ethics. However, it is limited to the theoretical and laboratory research due to the inability to solve the safety, efficiency and re-differentiation mechanism of induced pluripotent stem cells. Therefore, we are faced with enormous difficulties and challenges, which involve all aspects of basic research, including how to safely and effectively induce the differentiation of induced pluripotent stem cells into the desired cell type and how to establish a suitable disease model as well as a high-throughput drug screening platform.

关 键 词：干细胞 多能干细胞 组织工程 诱导 诱导多能干细胞 重编程 临床应用 遗传安全性 转录因子 疾病模型 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]