血管基质成分联合脱细胞骨基质-壳聚糖支架修复桡骨缺损  

Stromal vascular fraction combined with acellular bone matrix-chitosan scaffold for radical defect repair

作　　者：邵擎东[1] 汪铮[2] 李宇飞[3] 许天明[1] 孙久一[1] 江峰[1] 吕峰霞 

机构地区：[1]解放军第四五五医院骨科,上海市200052 [2]解放军第四五五医院生物诊疗科,上海市200052 [3]解放军第四五五医院整形外科,上海市200052

出　　处：《中国组织工程研究》2017年第6期843-847,共5页Chinese Journal of Tissue Engineering Research

基　　金：全军医药卫生科研项目(14ZD09)~~

摘　　要：背景:研究表明,复合使用人工组织工程骨材料和脱细胞骨基质做为骨缺损修复的支架材料,可以综合两者的优点。目的:进一步验证脂肪组织基质血管基质性成分联合脱细胞骨基质壳聚糖支架复合物修复兔桡骨缺损的安全性和生物相容性。方法:取38只新西兰大白兔,其中3只用于脂肪组织血管基质成分的制备,3只用于脱细胞骨基质的制备,32只分为实验组和对照组,采用Brownlow法制备桡骨缺损型,实验组移植血管基质成分-脱细胞骨基质壳聚糖复合支架,对照组造模后不做任何处理。术后2,4个月分别进行一般情况和大体观察、X射线拍照、病理观察、Lane-Sandhu组织学评分。结果与结论:1术后2,4个月2组无感染现象,但实验组的活动量和愈合程度显著性好于对照组;2术后2个月实验组骨缺损部位的X射线影像结果有显著灰白色的高密度影,术后4个月与正常的桡骨骨组织相同,对照组骨不连接;3术后2,4个月实验组病理显示骨组织生长情况显著性的高于对照组,Lane-Sandhu组织学评分显著性的高于对照组(P<0.05);4结果说明,血管基质成分-脱细胞骨基质壳聚糖复合支架修复兔桡骨缺损具有很好的安全性和生物相容性。BACKGROUND： Artificial tissue-engineered bone combined with acellular bone matrix has been shown to be favorable for bone repair.OBJECTIVE： To explore the safety and biocompatibility of the stromal vascular fraction of the adipose tissue combined with the acellular bone matrix-chitosan scaffold in the repair of rabbit radial defects. METHODS： A total of 38 New Zealand rabbits were selected, 3 rabbits were used to extract stromal vascular fraction of adipose tissue, 3 used to prepare acellular bone matrix and 32 divided into experimental and control groups. Models of rabbit radial defects were established using Brownlow method. The rabbits in the experimental group were treated with the SVF of adipose tissue combined with the acellular bone matrix-chitosan scaffold, while those controls received no treatment. General situation, gross observation, X-ray examination, histological observation and Lane-Sandhu scores were performed at 2 and 4 months postoperatively. RESULTS AND CONCLUSION： No infections occurred in both two groups at 2 and 4 months postoperatively, but the activity level and degree of healing in the experimental group were significantly better than those in the control group. In the experimental group, there were high-density shadows at 2 months postoperatively and the X-ray image of the bone defect site was the same as that of the normal one at 4 months, while bone nonunion occurred in the control group. The bone tissues in the experimental group grew significantly better than that in the control group at 2 and 4 months postoperatively, and the Lane-Sandhu histological scores in the experimental group were significantly higher than those in the control group at 2 and 4 months postoperatively（P〈0.05）. These results indicate that the stromal vascular fraction combined with the acellular bone matrix-chitosan scaffold exhibits safety and biocompatibility in the repair of rabbit radical defects.

关 键 词：骨基质 壳聚糖 支架 组织工程 生物材料 骨生物材料 血管基质成分 脱细胞骨基质 桡骨缺损 

分 类 号：R318[医药卫生—生物医学工程]