Bioaccumulation and the expression of hepatic cytochrome P450 genes in marine medaka(Oryzias melastigma)exposed to difenoconazole  

作　　者：Lemeng Zhang Xiaocui Dong Chonggang Wang Zhenghong Zuo Meng Chen 

机构地区：[1]Key Laboratory of Ministry of Education for Subtropical Wetland Ecosystem Research,Xiamen University,Xiamen 361102,China [2]State Key Laboratory of Marine Environmental Science,Xiamen University,Xiamen 361102,China

出　　处：《Journal of Environmental Sciences》2017年第2期98-104,共7页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.41376118)

摘　　要：This study was conducted to assess the effects of difenoconazole（DFZ）, a triazole fungicide,on the hepatic biotransformation system and its bioaccumulation in marine medaka（Oryzias melastigma）. Fish were exposed to DFZ（1, 10, 100, 1000 ng/L） for 180 days. The results showed that：（1） The m RNA levels of hepatic CYP1A1, CYP1 B, CYP1C1, CYP27 B and CYP3A40 were up-regulated, but those of CYP3A38 and CYP27A1 were down-regulated.（2） The activity of ethoxyresorufin-O-deethylase（EROD） and the content of reduced glutathione（GSH） in the liver were increased in the DFZ-treated groups, and glutathione S-transferase（GST） activity was increased in the 100 and 1000 ng/L groups.（3） DFZ was accumulated in the muscle and the biological concentration factors in the 10, 100, and1000 ng/L groups were respectively 149, 81 and 25. These results suggested that long-term exposure to DFZ at low concentrations would result in a bioaccumulation of this compound and disturb the biotransformation system.This study was conducted to assess the effects of difenoconazole（DFZ）, a triazole fungicide,on the hepatic biotransformation system and its bioaccumulation in marine medaka（Oryzias melastigma）. Fish were exposed to DFZ（1, 10, 100, 1000 ng/L） for 180 days. The results showed that：（1） The m RNA levels of hepatic CYP1A1, CYP1 B, CYP1C1, CYP27 B and CYP3A40 were up-regulated, but those of CYP3A38 and CYP27A1 were down-regulated.（2） The activity of ethoxyresorufin-O-deethylase（EROD） and the content of reduced glutathione（GSH） in the liver were increased in the DFZ-treated groups, and glutathione S-transferase（GST） activity was increased in the 100 and 1000 ng/L groups.（3） DFZ was accumulated in the muscle and the biological concentration factors in the 10, 100, and1000 ng/L groups were respectively 149, 81 and 25. These results suggested that long-term exposure to DFZ at low concentrations would result in a bioaccumulation of this compound and disturb the biotransformation system.

关 键 词：PESTICIDE DIFENOCONAZOLE Biotransformation system Marine fish 

分 类 号：X592[环境科学与工程—环境工程]