免疫层析试纸条技术及其在转基因检测中的应用  

Immunochromatography Test Strip and Its Applications in Detection of Genetically Modified Organisms

作　　者：夏启玉[1,2] 李美英[1,2] 杨小亮[1,2] 肖苏生[1,2] 贺萍萍[1,2] 郭安平[1,2] 

机构地区：[1]中国热带农业科学院热带生物技术研究所/农业部热带作物生物技术与遗传资源利用重点实验室,海口571101 [2]农业部转基因植物及植物用微生物环境安全监督检验测试中心,海口571101

出　　处：《中国生物工程杂志》2017年第2期101-110,共10页China Biotechnology

基　　金：公益性行业(农业)科研专项(201403075)资助项目

摘　　要：转基因检测是转基因生物安全监管的重要环节。目前需要一种适合于大规模筛查的快速检测转基因成分的方法,以扩大转基因生物安全的监测范围。免疫层析试纸条是一种快速免疫分析技术,是目前常用的快速检测方法之一,具有操作简单、样品量少、检测快速、成本低等优点,已在医学、食品和环境等多个领域广泛应用。介绍了免疫层析试纸条的组成结构、检测原理及免疫标记物,并总结了其在转基因检测方面的研究进展。Detection of genetically modified organisms（GMOs） is an important part of the safety supervision of GMOs. There is a need for a rapid method of detecting genetically modified components suitable for large-scale screening,in order to expand the scope of genetically modified organism ＇s safety monitoring.Immunochromatography test strip（ICTS） is a kind of fast immunoassay technology and it is one of the commonly used fast detection methods. Due to its simple operation,small quantity of sample,rapid detection,and low cost,etc., ICTS has been widely used in many fields, such as medicine, food and environment. The composition and structure,detection principle,and immune markers of ICTS were introduced,and its research progress in the detection of GMOs was summarized.

关 键 词：免疫层析试纸条 免疫标记物 转基因检测 蛋白试纸条 核酸试纸条 

分 类 号：Q789[生物学—分子生物学]