新西兰大白兔组织工程骨的构建及体内异位成骨效果  

The structure analysis of ectopic bone formed by engineered bone in the New-Zealand rabbits

作　　者：李海旭[1] 石继祥[1] 石文俊[1] 刘孚瑛[1] 章筛林[1] 周强[1] 

机构地区：[1]上海中医药大学附属普陀医院骨科,上海200062

出　　处：《中国临床医学》2017年第1期89-92,共4页Chinese Journal of Clinical Medicine

基　　金：上海市卫生和计划生育委员会科研课题(20114205)~~

摘　　要：目的:探讨新西兰大白兔组织工程骨股骨旁异位成骨(ectopic bone formation)的效果。方法:选取新西兰大白兔骨髓基质干细胞进行成骨诱导,与生物陶瓷构建组织工程骨,种植到新西兰大白兔股骨的骨膜旁,第8周获得异位成骨组织,检测分析收获的异位成骨材料结构。结果:第8周的异位成骨材料内有骨组织形成,成骨材料结构清晰,组织内部有新血管形成,荧光蛋白标记的标本有荧光蛋白表达。结论:组织工程骨在体内可顺利异位成骨。Objective：To study the structure of ectopic bone formed beside the New-Zealand rabbit femoral bone. Methods：Bone marrow stroma stem cells （BMSCs）were taken from New-Zealand rabbits to built engineered bone constructs, the constructs were embedded near the rabbit femoral bone,the ectopic bone constructs were harvested at 8^th week for further examination and study.Results：At 8th week,the ectopic bone had a clear bone-like structure,together with new blood supply, green fluorescent protein could be seen in samples seeded by marked cells.Conclusions：This study showed that the ectopic bone formation could be successfulinvivo.

关 键 词：组织工程骨 异位成骨 结构 

分 类 号：R683[医药卫生—骨科学]