灵芝三萜类化合物的体外抗肿瘤活性研究  

Study on in vitro anti-tumor activity of triterpenoids from Ganoderma lucidum

作　　者：闫征[1] 王宏旭[1] 刘莉莹[2] 杜国华[1] 陈若芸[2] 

机构地区：[1]中国医学科学院&北京协和医学院药物研究所新药作用机制研究与药效评价北京市重点实验室,北京100050 [2]中国医学科学院&北京协和医学院药物研究所天然药物活性物质与功能国家重点实验室,北京100050

出　　处：《国际检验医学杂志》2017年第5期633-634,637,共3页International Journal of Laboratory Medicine

摘　　要：目的研究灵芝三萜类化合物在不同肿瘤细胞上的抗肿瘤活性。方法四甲基偶氮唑盐(MTT)法检测3种灵芝三萜化合物对5种肿瘤细胞的抑制能力,绘制抑制曲线,并计算IC50,反映化合物的细胞毒活性。结果体外实验显示,3种灵芝三萜类化合物单体对5种肿瘤细胞表现出不同程度的抑制作用,其中灵芝酸Y的抑制作用比较强,对肺癌细胞H460的IC50为22.4μmol/L,其次是7-oxo-ganoderic acid Z2,IC50为43.1μmol/L。3种化合物灵芝酸Y、7-oxo-ganodericacid Z2和ganoderon B对其他被测细胞株未显示活性或活性很弱。结论灵芝酸Y对肺癌细胞H460显示了较强的抑制活性,7-oxo-ganoderic Z2对肺癌细胞H460显示了一定的抑制活性,且抑制作用具有剂量依赖性。灵芝酸Y和7-oxo-ganoderic Z2的抗肺癌作用有待于进一步深入研究。Objective To investigate the anti-tumor activity of triterpenoids from Ganoderma lucidum on different tumor cells.Methods The MTT assay was adopted to detect the in vitro inhibition effect on 5kinds of tumor cells.The inhibiting curve was drawn,IC50 was calculated for reflecting the compound′s cytotoxic activity.Results The in vitro experiments demonstrated that three kinds of triterpenoids compound monomer showed different degrees of inhibition effect,in which the inhibitory effect of ganoderenic acid Y was stronger,its IC50 on H460lung cancer cells was 22.4μmol/L,followed by 7-oxo-ganoderic acid Z2,its IC50 was43.1μmol/L.Conclusion Ganoderenic acid Y shows a strong inhibitory activity on H460 lung cancer cells,7-oxo-ganoderic acid Z2 shows a certain inhibitory activity on H460 lung cancer cells,moreover the inhibitory activity is dose dependent.The three compounds of ganoderenic acid Y,7-oxo-ganoderic acid Z2 and ganoderon B have no activity or very weak activity to the other detected cell lines.The anti-lung cancer activity of ganoderenic acid Y and 7-oxo-ganoderic acid Z2 needs to be further deeply studied.

关 键 词：灵芝子实体 三萜类化合物 抗肿瘤 四甲基偶氮唑盐 

分 类 号：R285[医药卫生—中药学]