两种方法检测146例抗核抗体结果的差异分析  

Analysis on results difference of two methods for detecting 146 cases of anti-nuclear antibody

作　　者：黄雪梅[1] 蒲泽晏 

机构地区：[1]四川省遂宁市中心医院检验科,62900

出　　处：《国际检验医学杂志》2017年第5期635-637,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨间接免疫荧光法(IIF)和免疫印迹法(LIA)检测抗核抗体(ANA)的结果差异及临床价值。方法收集146例ANA检测IIF(-)与LIA(+)的标本,并进行乙型肝炎病毒(HBV)抗原、抗体和丙型肝炎病毒(HCV)抗体的检测。结果 (1)在146例IIF(-)LIA(+)的标本中,单独出现抗Ro52抗体、抗SS-A、抗AMA-M2抗体阳性的标本数分别为69例、42例、15例,抗RNP抗体、抗PCNA抗体、PM-Scl抗体阳性的标本数分别为3例、5例、2例,有两项阳性的共8例。(2)单Ro52抗体阳性标本中,乙肝小三阳:HBsAg(+)、HBeAb(+)、HBcAb(+),乙肝大三阳:HBsAg(+)、HBeAg(+)、HBcAb(+)及丙型肝炎患者患者例数分别为51例、4例、7例,非肝炎患者7例。(3)单SSA抗体阳性标本中,乙型肝炎患者6例,非乙肝的患者36例。结论 IIF检测ANA容易漏检Ro52抗体、SSA抗体,Ro52抗体与乙肝小三阳有一定关系。Objective To investigate the results difference of the indirect immunofluorescence（IIF）and immunoblotting（LIA）for detecting ant-inuclear antibodies（ANA）and clinical value.Methods One hundred and forty-six ANA detection specimens of IIF negative and LIA positive were collected and performed the detection of HBV antibodies and anti-HCV antibodies.Results（1）In 146 specimens of IIF（-）LIA（＋）,the positive specimen numbers of single anti-Ro52 antibody,anti-SSA antibody and antiAMA-M2 antibody were 69 cases,42cases and 15 cases respectively,which of anti-RNP antibody,anti-PCNA antibody and PM-Scl antibody were 3cases,5cases and 2cases respectively,the combined 2-item positive was in 8cases.（2）Among positive specimens of single anti-Ro52 antibody,HBsAg（＋）,anti-HBe（＋）,anti-HBc（＋）model and HBsAg（＋）,HBeAg（＋）,HBcAb（＋）model of hepatitis B,and hepatitis C were 51 cases,4cases and 7cases respectively,7cases were non-hepatitis patients.（3）Among positive specimens of single ant-SSA antibody,6cases were hepatitis B patients and 36 cases were the patients with non-hepatitis B.Conclusion Anti-Ro52 antibody and anti-SSA antibody are easier to be missed by the IIF detection.Anti-Ro52 antibody positive has a certain relation with small three positive of hepatitis B.

关 键 词：抗Ro52抗体 抗SSA抗体 乙肝小三阳 抗核抗体 

分 类 号：R446.6[医药卫生—诊断学]