铅胁迫对百喜草叶绿素荧光特性及酶活性的影响  

Effect of lead stress on the chlorophyll fluorescence characteristics and antioxidative enzyme activities of Paspalum notatum

作　　者：侯晓龙[1,2,3] 蔡丽平[1,2] 韩航[1,2] 周垂帆[1,2] 王果[2] 刘爱琴[1,2] 

机构地区：[1]福建农林大学林学院,福建福州350002 [2]福建农林大学资源与环境学院,福建福州350002 [3]海峡两岸红壤区水土保持协同创新中心,福建福州350002

出　　处：《草业学报》2017年第3期142-148,共7页Acta Prataculturae Sinica

基　　金：国家科技支撑计划项目(2014BAD15B02);国家自然科学基金(41401364);国家林业局林业公益性行业科研项目(201304303);农业高校产学合作科技重大项目(2013N5002)资助

摘　　要：为探讨水土保持草种在重金属污染矿山废弃地植被恢复中应用的可行性,以南方地区水土流失治理中广泛应用的草种百喜草为试验材料,设置不同Pb浓度(150,250,500,1000,1500和2500mg/kg)的室内土培模拟胁迫试验,定量测定不同Pb胁迫浓度、不同胁迫时间百喜草的叶绿素荧光、SPAD值、叶绿素含量、抗氧化酶活性及MDA含量等指标,分析百喜草对Pb胁迫的生理响应。结果表明,在试验初期(8月),Pb胁迫对百喜草的叶绿素荧光特性具有一定抑制作用,但随胁迫时间的增加,在试验后期(9和10月)则表现出显著的促进作用(P<0.05);Pb胁迫对百喜草SPAD值和叶绿素含量有一定抑制作用,但作用并不显著(P>0.05);Pb胁迫对百喜草的POD和SOD活性均具有一定促进作用,但对百喜草CAT活性无显著影响(P>0.05);铅胁迫增大百喜草体内MDA含量,但高浓度Pb胁迫(1500和2500mg/kg)条件下,百喜草体内MDA含量与对照无显著差异(P>0.05)。以上结果表明,Pb胁迫对百喜草生理特性有一定抑制作用,但抑制作用并不显著。百喜草可通过提高叶绿素荧光特性和抗氧化酶(POD和SOD)活性,减轻Pb胁迫的危害,以适应Pb胁迫逆境环境。Paspalum notatum is considered one of the best grass species for controlling soil and water loss in south China. To test the feasibility of P. notatum as a restoration plant in mining wasteland, a pot experiment was undertaken with various concentrations of lead （Pb） （150, 250, 500, 1000, 1500 and 2500 mg/kg）. Chlo-rophyll fluorescence, SPAD value, chlorophyll content, antioxidative enzyme activities and malondialdehyde content were measured to investigate physiological responses to Pb stress. The inhibitory effect of Pb stress on chlorophyll fluorescence was observed at the early stage （August） , while significant promotion was found at later stages （September and October）. Pb stress inhibited the SPAD value of chlorophyll and the chlorophyll content of P. notatum^ but no significant differences from the control were observed. Pb stress promoted the activities of peroxidase and superoxide dismutase, but showed little effect on catalase activity. Malondialdehyde content of P . notatum increased under Pb stress, but there were no significant differences from the control at higher Pb concentrations. These results demonstrate that Pb stress inhibits some physiological characteristics in P . notatum and that the antioxidant system and chlorophyll fluorescence may play important roles in allevia-ting Pb toxicity.

关 键 词：百喜草 PB胁迫 叶绿素 抗氧化酶 丙二醛 

分 类 号：Q945.78[生物学—植物学]