CGRP对大鼠急性心肌梗死后心脏干细胞募集及心功能的影响  

Effect of calcitonin gene-related peptide on recruitment of cardiac stem cells and cardiac functions after acute myocardial infarction in rats

作　　者：曹桎铭 刘志江[2] 王艳[2] 陈文明[2] 石蓓[2] CAO Zhi-ming LIU Zhi-jiang WANG Yan CHEN Wen-ming SHI Bei(Department of Cardiology, Anyue People’s Hospital, Anyue 642350, Sichuan, China Department of Cardiology, Affiliated Hospital to Zunyi Medical College, Zunyi 563003, Guizhou, China)

机构地区：[1]四川省安岳县人民医院心血管内科,四川安岳642350 [2]遵义医学院附属医院心血管内科,贵州遵义563003

出　　处：《心脏杂志》2017年第2期158-163,共6页Chinese Heart Journal

基　　金：国家自然科学基金项目资助(81360021)

摘　　要：目的观察降钙素基因相关肽(calcitonin gene related peptide,CGRP)对心脏干细胞(cardiac stem cells,CSCs)募集及心功能的影响。方法建立大鼠急性心肌梗死(AMI)模型,随机分为假手术组、CGRP注射组(CGRP组)和等体积磷酸盐缓冲液(PBS)注射组(对照组),每组30只。模型建立后于不同时间点获取标本,采用免疫荧光染色法观察CSCs募集情况,超声心动图评估大鼠心肌梗死后心功能,苏木素-伊红(H-E)染色、Masson染色评估心脏病理学改变,2,3,5-氯化三苯基四氮唑(TTC)染色测定心肌梗死面积。结果免疫荧光染色显示,与假手术组相比,对照组和CGRP组CSC细胞数均在第3天增加,7 d达高峰,14 d明显减少,其中,CGRP组较对照组增加更明显(均P<0.05)。术后28 d大鼠超声心动图检测大鼠心功能提示CGRP组较对照组心功能各项指标明显改善(均P<0.05),形态学分析显示术后28 d CGRP组心肌纤维化程度及梗死面积明显小于对照组(P<0.05)。结论CGRP可促进AMI后CSC在梗死区域募集,于第7天达高峰,并能改善大鼠心功能。AIM To observe the effect of calcitonin gene-related peptide （CGRP） on cardiac stem cell recruitment and cardiac function after acute myocardial infarction （AMI） in rats. METHODS AMI rat model was established by left anterior descending artery ligation. Rats were randomly divided into sham-operated group （n=30）, control group （n=30） and CGRP group （n=30）. Phosphate-buffered saline （PBS） in control group or CGRP in CGRP group was injected into rat cardiac tissues after MI. Animals were sacrificed at 3 days, 7 days, 14 days and 28 days. Recruitment of cardiac stem cells （CSCs） was observed by immunofluorescence staining, cardiac morphological changes were observed by HE, Masson and TTC staining, and cardiac functions were assessed using echocardiography. RESULTS The number of CSCs increased on day 3, reached the peak on day 7 and decreased significantly on day 14 in both control group and CGRP group. CSCs significantly increased in control group at each time point compared with those in sham-operated group （P〈0.05）. CSCs in CGRP group were more obviously increased compared with those in control group （P〈0.05）. At 28 days after operation, cardiac function indexes were significantly improved, whereas collagen fibers and infarct area were significantly reduced in CGRP group compared with those in the control group （both P〈0.05）. CONCLUSION CGRP could promote CSC recruitment in the infarcted zone, which reaches the peak on day 7. GGRS improves cardiac functions after AMI.

关 键 词：降钙素基因相关肽 心脏干细胞 心肌梗死 心功能 

分 类 号：R542.2[医药卫生—心血管疾病]