脂肪源性干细胞促进大鼠受损坐骨神经传导及脊髓脑源性神经营养因子和睫状神经营养因子的表达  

Adipose derived stem cells promote nerve conduction of rat injured sciatic nerve and expression of brain-derived neurotrophic factor and ciliary neurotrophic factor in spinal cord

作　　者：付秀美[1] 杨海艳 王荣良 杨振江[1] 付文亮[1] 薛景凤[1] Fu Xiumei Yang Haiyan Wang Rongliang Yang Zhenjiang Fu Wenliang Xue Jingfeng(Department of Human Anatomy, Chengde Medical College Chengde Traditional Chinese Medicine Hospital Chengde Maternal and Child Health Hospital, Chengde 067000, China)

机构地区：[1]承德医学院人体解剖学教研室 [2]承德市中医院 [3]承德市妇幼保健医院,承德067000

出　　处：《解剖学杂志》2017年第1期11-15,共5页Chinese Journal of Anatomy

基　　金：河北省教育厅基金(QN2014138)

摘　　要：目的:探讨脂肪源性干细胞(ADSCs)对坐骨神经损伤大鼠神经传导功能以及脊髓脑源性神经营养因子(BDNF)和睫状神经营养因子(CNTF)表达的影响。方法:将第4代ADSCs移植入脱细胞神经移植物(ANA)中,构建组织工程神经。大鼠随机分为正常组、杜氏改良Eagle培养基营养混合物F12(DMEM)组和ADSC组。DMEM组和ADSC组均建立坐骨神经损伤模型,后用相应的组织工程神经桥接损伤神经的断端。术后6周采用神经电生理记录仪检测各组大鼠坐骨神经传导速度和波幅,采用免疫荧光和Real-time PCR检测各组大鼠脊髓脑源性神经营养因子(BDNF)、睫状神经营养因子(CNTF)蛋白和mRNA的表达。结果:ADSC组大鼠坐骨神经传导速度、波幅和脊髓BDNF和CNTF蛋白及mRNA表达均显著高于DMEM组。结论:ADSCs可增加坐骨神经传导速度和波幅、上调脊髓BDNF和CNTF的表达。Objective： To investigate the effects of adipose derived stem cells （ADSCs） on the function of nerve conduction and the expression of brain-derived neurotrophic factor （BDNF） and ciliary neurotrophic factor （CNTF） in spinal cord of rats with sciatic nerve injury. Methods： ADSCs within passage 4 were transplanted into the acellular nerve graft （ANA）, and the tissue engineering nerves were constructed. The rats were randomly divided into 3 groups： normal group, DMEM group and ADSC group. Sciatic nerve injury model was established in rats of DMEM group and ADSC group, then the corresponding tissue engineering nerves were used to bridge the injured sciatic nerves, six weeks after operation, the sciatic nerve conduction velocity and amplitude were detected by nerve electrophysiological recording instrument. Immunofluorescence and real-time PCR were used to detect the expression of BDNF and CNTF protein and mRNA in spinal cord. Results： In contrast with DMEM group, the sciatic nerve conduction velocity and amplitude, the expressions of BDNF and CNTF protein and mRNA in ADSC group rats increased remarkably. Conclusion： ADSCs can increase the sciatic nerve conduction velocity and amplitude and increase the expression of BDNF and CNTF in the spinal cord of rats with sciatic nerve injury.

关 键 词：脂肪源性干细胞 大鼠 脊髓 脑源性神经营养因子 睫状神经营养因子 

分 类 号：R651.3[医药卫生—外科学]