快律宁胶囊及其含药血清对大鼠心室肌细胞钙离子浓度的影响  

Influence of Kuailvning capsule and its drug serum on the concentration of Ca^2 ＋ in rat ventricular muscle cells

作　　者：胡华杰 郭燕娜 温慧萍[2] Hu Huajie Guo Yanna Wen Huiping(Department of Pharmacy, the Traditional Chinese Medicine Hospital of Zhoushan , Zhoushan , Zhejiang 316000, China Lishui College,, Lishui, Zhejiang 323000, China)

机构地区：[1]舟山市中医院药剂科,浙江省舟山316000 [2]丽水学院,浙江省丽水323000

出　　处：《中国基层医药》2017年第6期886-890,共5页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的：研究快律宁胶囊（KLN）含药血清对大鼠心室肌细胞钙离子（Ca^2＋）浓度的影响。方法将 SD 大鼠采用数字表法随机分为空白对照组、血清对照组、KLN 高、中、低剂量组，每组各6只，用酶解法急性分离大鼠心室肌细胞，采用 Fluo-3／AM荧光探针测定不同浓度的 KLN 及其含药血清对单个大鼠心室肌细胞内 Ca^2＋浓度的影响，比较不同组别 Ca^2＋浓度。结果与对照组比较，KLN 高、中、低剂量组在50 s、100 s、150 s、200 s、250 s 后胞浆 Ca^2＋平均荧光强度分别为[（18．75±1．55）、（16．69±0．93）、（17．76±1．26）]，[（25．47±1．118）、（17．86±1．49）、（17．81±1．13）]，[（29．05±1．31）、（20．14±1．73）、（18．26±1．37）]，[（35．21±1．33）、（23．19±0．97）、（18．18±1．46）]，[（41．08±1．21）、（26．34±1．69）、（17．91±1．01）]，均明显高于对照组（F ＝5．556、7．007、8．816、10．208、12．232，均 P ＜0．01）。与对照组相比，KLN 高剂量含药血清组50 s、100 s、150 s、200 s、250 s 后胞浆 Ca^2＋平均荧光强度分别为（17．85±1．69）、（18．95±1．73）、（21．85±1．39）、（23．01±1．48）、（24．07±1．42），明显高于血清对照组（t ＝3．642、4．406、6．074、7．402、8．625，P ＜0．01），中剂量含药血清组100 s、150 s、200 s、250 s 后胞浆 Ca^2＋平均荧光强度分别为（18．01±1．42）、（18．76±1．22）、（19．73±1．37）、（21．04±1．16），明显高于血清对照组（t ＝3．902、4．033、4．126、4．326，均 P ＜0．01），而 KLN 低剂量含药血清组对 Ca^2＋平均荧光强度的影响差异无统计学意义（P ＞0．05）。结论 KLN 含药血清能促进心肌细胞自发的外钙内流，说明 KLN 含药血清具有抗心律作用且其与对 Ca^2＋通道的作用有关。Objective To observe the influence of Kuailvning capsule（KLN）and its drug serum on the concentration of Ca^2＋ in rat ventricular muscle cells.Methods SD rats were randomly divided into five groups：the control group,serum control group,KLN high -dose group,KLN medium -dose group and KLN low -dose group,six mice in each group.The rat ventricular myocytes were separated by enzymatic hydrolysis.The influence of KLN and its drug serum on the concentration of Ca^2＋ in rat ventricular muscle cells were observed by Fluo -3 /AMprobe.Results The mean fluorescence intensity of cytosolic Ca^2＋ in the KLN high -dose group,KLN medium -dose group and KLN low -dose group after 50s,100s,150s,200s,250s were [（18.75 ±1.55）,（16.69 ±0.93）,（17.76 ±1.26）], [（25.47 ±1.118）,（17.86 ±1.49）,（17.81 ±1.13）],[（29.05 ±1.31）,（20.14 ±1.73）,（18.26 ±1.37）], [（35.21 ±1.33）,（23.19 ±0.97）,（18.18 ±1.46）],[（41.08 ±1.21）,（26.34 ±1.69）,（17.91 ±1.01）], which were higher than those in the control group（F =5.556,7.007,8.816,10.208,12.232,all P 〈0.01 ）.The mean fluorescence intensity of cytosolic Ca^2＋ in the KLN high -dose group after 50s,100s,150s,200s,250s were （17.85 ±1.69）,（18.95 ±1.73）,（21.85 ±1.39）,（23.01 ±1.48）,（24.07 ±1.42）,which were higher than those in the serum control group（t =3.642,4.406,6.074,7.402,8.625,all P 〈0.01 ）.The mean fluorescence intensity of cytosolic Ca^2＋ in the KLN medium -dose group after 100s,150s,200s,250s were （18.01 ±1.42）, （18.76 ±1.22）,（19.73 ±1.37）,（21.04 ±1.16）,which were higher than those in the serum control group（t =3.902,4.033,4.126,4.326,all P 〈0.01）.There were no statistically significant difference between the KLN low -dose group and the serum control group（P 〉0.05）.Conclusion KLN drug serum can promote myocardial cell spon-taneous extracellular calcium influx,the mechanism of KLN antiarrhythmic effect may be related to the Ca^2＋ channel.

关 键 词：医院制剂 快律宁胶囊 心室肌细胞 细胞内钙浓度 

分 类 号：R285.5[医药卫生—中药学]