多指标优选蛭丹化瘀组方的水提醇沉工艺  

Optimization of Water Extraction and Alcohol Precipitation Technology for Zhidanhuayu Formula with Multi-index

作　　者：张欣[1] 倪健[1] 张玲玲[1] 张娟[1] 王海涛[1] 杨春静[1] 李任[2] 沈素[2] 尹兴斌[1] ZHANG Xin NI Jian ZHANG Ling-ling ZHANG Juan WANG Hai-tao YANG Chun-jing LI Ren SHEN Su YIN Xing-bin(Chinese Medicine School of Beijing University of Chinese Medicine, Beijing 100102, China Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China)

机构地区：[1]北京中医药大学中药学院,北京100102 [2]首都医科大学附属北京友谊医院,北京100050

出　　处：《现代中药研究与实践》2017年第1期44-48,共5页Research and Practice on Chinese Medicines

基　　金：北京市科学技术委员会"十病十药"研发项目"蛭丹化瘀组方用于治疗小儿病毒性肺炎的成药性研究"(Z141100002214019)

摘　　要：目的优选蛭丹化瘀组方的水提醇沉工艺。方法以干膏得率为参考,以芍药苷、黄芪甲苷含量为指标,采用L_9(3~4)正交试验优选出最佳的水提工艺,采用单因素试验优选出最佳的醇沉工艺。结果优选出的工艺为8倍量水煎煮3次,每次1 h,提取液滤过,合并滤液,浓缩至相对密度为1.10~1.15(60℃)的药液,冷却至室温,加入适量乙醇使醇浓度达60%。醇沉后,干膏得率减少约5%。结论优选出的水提醇沉工艺条件稳定性好,合理可行,可为大生产提供参考。Objective The technique of water extraction and alcohol precipitation of Zhidanhuayu Formula was optimized.Methods With dry extract yield as the reference,Paeoniflorin and Astragaloside IV extraction contents as indexes,L9（3^4） orthogonal test was adopted to optimize the best water extraction process,the single factor test was adopted to optimize the best alcohol precipitation process.Results Optimal process was：extracted 3 times with 8 folds of the amount of water,1 hour for each time.The extracts were filtered,combined and concentrated to the relative density of 1.10～1.15（60℃）,cooled to room temperature.Add suitable amount of alcohol to attain to the alcohol concentration of 60%.After alcohol precipitation,dry extract yield decreased about5%.Conclusion The optimized condition of water extraction and alcohol precipitation was of good stability,reasonable and feasible,which can provide a reference for the mass production.

关 键 词：多指标 正交试验 水提醇沉 芍药苷 黄芪甲苷 

分 类 号：R283[医药卫生—中药学]