苍耳子水提取液对小鼠肝毒性“量-时-毒”关系研究  

Study on "dose-time-toxicity" relationship on hepatotoxicity caused by water extract of Xanthium in mice

作　　者：汪永忠[1] 洪燕[2] 李钰馨 韩燕全[1] Wang Yongzhong Hong yan Li Yuxin Han Yanquan(Grade 3 Laboratory of Traditional Chinese Medicine Preparation,State Administration of Traditional Chinese Medicines, The First Affiliated Hospital,Anhui University of Traditional Chinese Medicine,Hefei Anhui 230031 Anhui University of Traditional Chinese Medicine, Hefei A nhui 230012)

机构地区：[1]安徽中医药大学第一附属医院国家中医药管理局中药制剂三级实验室,安徽合肥230031 [2]安徽中医药大学,安徽合肥230012

出　　处：《山西中医学院学报》2016年第6期1-4,共4页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：国家中医药管理局中药炮制技术传承基地项目

摘　　要：目的:考察苍耳子水提物单次给予对小鼠肝毒性的"量-时-毒"关系。方法:将小鼠按不同时间点和不同剂量分组,观察小鼠急性肝毒性反应,检测血清谷氨酸氨基转移酶(ALT)、谷草氨酸氨基转移酶(AST)水平,计算肝体比值,光学显微镜下观察肝组织病理形态变化。结果:"时-毒"关系研究表明:制备4.0 g/m L苍耳子水提液,取小鼠按不同时间点分组,按101.14 g/kg剂量小鼠灌胃给药一次,分别于给药后各时间点取血,小鼠血清ALT,AST均在给药1 h后明显升高,给药后8 h达到高峰,24 h后恢复近正常值;肝体比值增大,肝组织病理损伤严重;"量-毒"关系研究表明:小鼠单次灌胃给药不同剂量(101.14 g/kg、83.312 g/kg、66.650 g/kg、53.320 g/kg、42.656 g/kg、空白组同体积蒸馏水)。苍耳子水提液对肝组织产生明显损伤,随剂量增大,血清ALT,AST值显著升高。肝体比值增大,肝组织病理损伤严重。结论:小鼠单次灌胃给药苍耳子水提取液可造成明显急性肝损伤,并呈现一定"量-时-毒"关系。Objective：To study the ＂dose-time-toxicity＂ relationship of hepatotoxicity in mice caused by single dosage water extract of Xanthium. Method：Mice were packeted according to different time and dose points,to observe the hepatotoxicity of mice,detect the ALT、AST in serum,calculate liver index,and observe the histopathology of liver tissue.Result：The ＂time-toxicity＂ relationship showed that：administrated the 101.14 g/kg water extract of Xanthium to mice,take the blood samples at different time after administration,ALT and AST were increased after 1 hour,peaked at 8 hour and turned back to normal level at 24 hour;liver index increased and histopathological examination showed serious injury of liver tissue. The ＂dosage-toxicity＂ relationship showed that：the dosage ranger of water extract of Xanthium cause injury is101.14 g/kg、83.312 g/kg、66.650 g/kg、53.320 g/kg、42.656 g/kg,the ALT and AST increased significantly as the dosage increased.Live index also increased,live tissue histopathology serious injury was observed. Conclusion：The acute liver injury can be caused by single dose water extract of Xanthium to mice,and there are certain ＂time-dosage-toxicity＂ relationship.

关 键 词：苍耳子水提液 肝毒性 时毒 量毒 

分 类 号：R285[医药卫生—中药学]