2011—2014年江西地区猪繁殖与呼吸综合征病毒的分子流行病学调查  

Molecular Epidemiology of Porcine Reproductive and Respiratory Syndrome Virus In Jiangxi Province during 2011-2014

作　　者：江松泽 汪志[2] 师东方[3] 张桂红[2] JIANG Songze WANG Zhi SHI Dongfang ZHANG Guihong(Harbin Weike Biotechnology Development Company, Harbin 150069, China Veterinary College, South China Agricultural University, Guangzhou 510642, China Northeast Agricultural University, Harbin 150030, China)

机构地区：[1]哈尔滨维科生物技术开发公司,黑龙江哈尔滨150069 [2]华南农业大学兽医学院,广东广州510642 [3]东北农业大学,黑龙江哈尔滨150030

出　　处：《广东畜牧兽医科技》2016年第6期34-37,43,共5页Guangdong Journal of Animal and Veterinary Science

基　　金：公益性行业(农业)科研专项经费(201203039);国家生猪产业技术体系建设专项(CARS-36)

摘　　要：为了研究江西地区2011—2014年猪繁殖与呼吸综合征的流行情况,收集江西11个市的2475份猪血清样本,采用ELISA试剂盒检测血清中的PRRSV抗体水平。在2475份血清中选取8份(每年份2个)通过RT-PCR的方法扩增ORF5序列,克隆、测序后,用DNAstar和Mega5.0软件对所得序列进行遗传进化分析。结果表明,2011—2014年江西省11个市猪蓝耳病免疫抗体水平较高;所选取8份血清均为阳性,检测的PRRSV毒株均属于美洲型毒株,均为高致病性毒株,各毒株间核苷酸同源性为96.4%-99.3%,与PRRSV高致病性变异毒株的同源性最高,表明2011—2014年流行的PRRSV可能为猪高致病性蓝耳变异毒株。Porcine sera were collected from 11 city zones of Jiangxi province, which were used for investigating the epidemiology.Then the ELISA kit was used to test serum antibody levels.In order to further reveal the possible prevalence of PRRSV in Jiangxi, 8 sera were chosen from the 2475 sera（2 sera per year）.The complete ORF5 gene were amplified by RT-PCR.Then the sequence comparison and genetic phylogenetic analysis were conducted by DNAStar and Mega 5.0.The results showed that the city zones of Jiangxi province tested had high serum antibody levels during 2011—2014.All the 8 virus strains were positive samples and belonged to American genotype and they were highly pathogenic PRRSV.The homologies of ORF5 genes among the 8 PRRSV was 96.4%-99.3%.They had the highest homologies with the highly pathogenic mutations of PRRSV, which indicated that the epidemic PRRSV in Jiangxi province may be highly pathogenic mutations of PRRSV during 2011—2014.

关 键 词：猪繁殖与呼吸综合征 遗传进化分析 血清学调查 ORF5基因 分子流行病学 

分 类 号：S851.3[农业科学—预防兽医学]