新生期间断重复吸入七氟醚对大鼠学习记忆及Tau蛋白和p-Tau蛋白的影响  

Effects of neonatal repeated intermittent inhalation of sevoflurane on learning-memory and Tau protein,p-Tau protein in juvenile and adult rats

作　　者：王权 张超 王义 朱昭琼 

机构地区：[1]贵州省麻醉与器官保护基础研究重点实验室,遵义医学硕士研究生563000

出　　处：《医学研究生学报》2017年第3期257-261,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81360180)

摘　　要：目的学习记忆功能是大脑的高级神经活动形式,一旦大脑海马组织受损,可严重影响患者的生活质量。文中旨在观察新生期大鼠间断重复吸入七氟醚对其幼年期、成年期学习记忆功能以及大脑海马中Tau蛋白、p-Tau蛋白的影响。方法 24只健康新生SD大鼠,采用随机数字表法分为幼年期七氟醚吸入组、幼年期对照组、成年期七氟醚吸入组和成年期对照组,每组6只。幼年期七氟醚吸入组、成年期七氟醚吸入组分别于出生后第7、14、21天(记录P7、P14、P21)吸入2.6%七氟醚2h,幼年期对照组、成年期对照组则在相同的时间点吸入运载气体(1L/min O2+1 L/min Air)2 h。幼年期七氟醚吸入组与幼年期对照组于P31-37,成年期七氟醚吸入组与成年期对照组于P91-97行Morris水迷宫实验检测其行为学,然后取其海马组织,检测海马中Tau蛋白与p-Tau蛋白表达水平。结果幼年期和成年期七氟醚吸入组与其对照组逃避潜伏期同时间点比较差异无统计学意义(P>0.05)。空间探索实验结果比较:幼年期与成年期大鼠在原平台区域进入次数、游泳滞留时间、游泳路程以及游泳速度等组间比较差异均无统计学意义(P>0.05)。Tau蛋白表达检测:与幼年期对照组海马CA1、CA3、DG区表达(0.185±0.024、0.232±0.040、0.184±0.018)相比,幼年期七氟醚吸入组(0.237±0.015、0.324±0.024、0.226±0.019)明显增高(P<0.01);成年期七氟醚吸入组与其对照组比较差异无统计学意义(P>0.05)。p-Tau蛋白表达检测:与成年期对照组海马CA3区表达(0.158±0.011)相比,成年期七氟醚吸入组表达水平(0.170±0.005)增高(P<0.05);而幼年期七氟醚吸入组与其对照组比较差异无统计学意义(P>0.05)。结论新生期间断重复吸入七氟醚麻醉未见损害大鼠在幼年期和成年期的学习记忆功能,推测其可能与幼年期增加的Tau蛋白和成年期增加的p-Tau蛋白表达并不足以引起其学习记忆功能下�Objective Learning and memory function is the form of brain higher nervous activity. Hippocampus is the main parts responsible for learning and memory function. Once damaged, it will seriously affect the quality of life in patients. The purpose of this paper was to observe the effects of neonatal repeated intermittent sevoflurane inhalation on learningmemory function and Tau protein, p-Tau protein in brain hippocampus in juvenile and adult rats. Methods Twenty-four healthy SD rats （n = 2 4 ） were randomly divided into juvenile sevoflurane inhalation group （n = 6 ） , juvenile control group （n = 6） ; adult sevoflurane inhalation group （n = 6） , and adult control group （n = 6） . Rats in juvenile sevoflurane inhalation group and adult sevoflurane inhalation group inhaled 2.6% sevoflurane at the postnatal 7th day, 14th day, 21th day （P7,P14,P21） for 2 hours. Rats in juvenile control group and adult control group inhaled the carrier gas （ 1 L/min Air ＋ 1 L/min 02） at the same time for 2 hours. During P31 ？37 ,Morris water maze test was conducted in juvenile sevoflurane inhalation group and juvenile control group to detect the behavior. During P91 - 97,Morris water maze test was conducted in adult sevoflurane inhalation group and adult control group to detect the behavior. Then hippocampi were taken out to detect the expression levels of Tau protein and p-Tau protein. Results （1） Comparison of escape latency at the same time ① Juvenile period ： no statistical difference between sevoflurane inhalation group （52.04±41.90,29.77±14.23, 19.87±5.71,22.74± 13.73,21.91 ±9.07） and control group （47.82±8.06,25.26±12.53,23.79±9.49, 20.00±10.10,14.03±7.55） had （P〉0.05）.② Adult period： no statistical difference between sevoflurane inhalation group（42.00± 14.12, 26.87±16.93, 19.80±13.76, 15.06±8.45, 8.66±4.82） and control group （41.97±25.66,22.88±10.04, 15.8 8± 5

关 键 词：七氟醚 海马 学习记忆 TAU蛋白 p-Tau蛋白 

分 类 号：R971.2[医药卫生—药品]