朱顶红的组培快繁技术  

Study on Tissue Culture and Rapid Propagation Techniques for Amaryllis vittata

作　　者：陈汉鑫 余松金 万学锋 郑春生 陈前程 杨宗锦 CHEN Han-xin YU Song-jin WAN Xue-feng ZHENG Chun-sheng CHEN Qian-cheng YANG Zong-jin(Zhangzhou Agriculture Science Institute ,Zhangzhou 363005 ,Fujian ,Chin)

机构地区：[1]福建省漳州市农业科学研究所,福建漳州363005

出　　处：《福建林业科技》2016年第3期170-173,186,共5页Journal of Fujian Forestry Science and Technology

基　　金：漳州市农科所自然基金项目(ZN201402)

摘　　要：以朱顶红鳞茎盘为外植体,开展组培快繁技术研究。结果表明:最佳诱导培养基为MS+6-BA 2.0 mg·L^(-1)+NAA0.1 mg·L^(-1)+蔗糖30 g·L^(-1)+琼脂6.0 g·L^(-1),诱导率为64.3%;合适的增殖培养基为MS+6-BA 2.0 mg·L^(-1)+NAA0.2 mg·L^(-1)+蔗糖30 g·L^(-1)+琼脂6.0 g·L^(-1),增殖系数达5.62倍;合适的生根培养基为:1/2 MS+IBA 0.5 mg·L^(-1)+NAA 0.5 mg·L^(-1)+蔗糖20 g·L^(-1)+琼脂6.0 g·L^(-1),生根率达90.0%;适宜的移栽基质为:泥炭土∶园土∶珍珠岩(体积比)=5∶1∶1,移栽成活率可达93.6%。The tissue culture for Amaryllis vittata was conducted using its basal bulbs as explants. The results showed that the optimal shoot - inducing medium was MS ＋ 6-BA 2. 0 mg · L ^-1 ＋ NAA 0. 1 mg· L ^-1 ＋ sucrose 30 g · L ^-1 ＋ agar 6. 0 g· L ^-1, with inducement rate up to 64. 3%. The optimal shoots muhiplication culture medium was MS ＋ 6-BA 2. 0 mg ~ L-1 ＋ NAA 0. 2 mg· L ^-1 ＋ sucrose 30 g· L ^-1 ＋ agar 6. 0 g · L ^-1 ,with multiplication coefficient of 5.62 times. The optimal rooting culture medium was 1/2 MS ＋ IBA 0. 5 mg · L ^-1 ＋ NAA 0. 5 mg · L ^-1 ＋ sucrose 20 g · L ^-1＋ agar 6.0 g· L ^-1 ,with rooting rate up to 90. 0%. The optimal transplant medium was peat soil： garden soil： perlite （V/V） = 5：1：1, with survival rate up to 93.6% .

关 键 词：朱顶红 鳞茎盘 组织培养 

分 类 号：S682.25[农业科学—观赏园艺]