过表达N-myc下游调节基因2(NDRG2)抑制直肠癌细胞的增殖和迁移并促进细胞凋亡  

Overexpression of N-myc downstream regulated gene 2(NDRG2) inhibits proliferation,migration and promotes apoptosis in SW480 rectal cancer cells

作　　者：李志强[1] 孙洋[1] 万鸿兴 柴芳[1] 

机构地区：[1]海南省三亚市人民医院肿瘤科,海南三亚572000

出　　处：《细胞与分子免疫学杂志》2017年第1期48-52,共5页Chinese Journal of Cellular and Molecular Immunology

摘　　要：目的探讨N-myc下游调节基因2(NDRG2)基因对直肠癌细胞增殖、迁移、凋亡的影响。方法培养SW480人直肠癌细胞,以p CDNA3.1为载体,将p CDNA3.1-NDRG2和空载体(SW480-Ve)转染至SW480人直肠癌细胞,并设定SW480细胞为空白对照组。MTT法检测SW480、SW480-Ve、SW480-NDRG2组细胞增殖活性;划痕愈合试验检测三组细胞在24、48、72 h迁移距离;流式细胞术检测三组转染48 h细胞凋亡率;Western blot法检测三组细胞Bax、caspase-3、Bcl-2蛋白表达。结果细胞培养7 d后,SW480-NDRG2组细胞生存率显著低于SW480组和SW480-Ve组,且细胞生存率随着培养时间延长逐渐降低,而SW480-Ve组细胞生存率与SW480组无显著差异;SW480-NDRG2组细胞迁移距离显著低于SW480-Ve组和SW480组,SW480-Ve组和SW480组细胞迁移距离无显著差异;SW480-NDRG2组细胞凋亡率显著高于SW480组和SW480-Ve组,SW480组和SW480-Ve组细胞凋亡率差异不明显;SW480-NDRG2组细胞Bax和caspase-3蛋白表达量显著高于SW480组和SW480-Ve组,Bcl-2蛋白表达量显著低于SW480细胞和SW480-Ve细胞,SW480组和SW480-Ve组Bax、caspase-3和Bcl-2蛋白表达差异不明显。结论过表达NDRG2抑制人直肠癌细胞株SW480的增殖,降低细胞迁移,通过调控Bcl-2、Bax和caspase-3蛋白表达促进细胞凋亡。Objective To investigate the role of N-myc downstream regulated gene 2（ NDRG2） gene in the proliferation,migration and apoptosis of rectal cancer cells. Methods Human rectal cancer SW480 cells were cultured and transfected with p CDNA3. 1-NDRG2 and empty vector（ SW480-Ve）. SW480 cells were set as a control group. Cell proliferation was detected in SW480 cells,SW480-Ve cells and SW480-NDRG2 cells by MTT assay; cell migration distance in the three groups at 24,48,72 hours was tested by wound healing assay; apoptosis rate was determined in the three groups at 48 hours by flow cytometry; the expressions of Bax,caspase-3,Bcl-2 proteins in the three groups were examined by Western blotting.Results After the cells were cultured for 7 days,cell survival rate in SW480-NDRG2 group was significantly lower than that in SW480 cells and SW480-Ve cells; the cell survival rate decreased gradually with the prolongation of the culture time; and it had no significant difference between SW480-Ve group and SW480 group. Cell migration distance in SW480-NDRG2 group was significantly lower than that in SW480-Ve cells and SW480 cells,and it had also no significant difference between SW480-Ve cells and SW480 cells. The apoptosis rate in SW480-NDRG2 group was significantly higher than that in SW480 group and SW480-Ve group,and SW480 cells and SW480-Ve cells had no significant difference in the rate. The expressions of Bax and caspase-3 proteins in SW480-NDRG2 group were significantly higher than those in SW480 cells and SW480-Ve cells; Bcl-2protein expression was significantly lower in SW480-NDRG2 group than in SW480 cells and SW480-Ve cells; and the expressions of Bax,caspase-3 and Bcl-2 proteins were not significantly different between SW480 cells and SW480-Ve cells.Conclusion Overexpression of NDRG2 can inhibit the proliferation,reduce cell migration,and promote cell apoptosis by regulating the expressions of Bcl-2,Bax and caspase-3 proteins in SW480 cells.

关 键 词：N-myc下游调节基因2(NDRG2) 直肠癌 SW480细胞 增殖 迁移 凋亡 

分 类 号：R392-33[医药卫生—免疫学]