胃腺癌患者外周血PD-1^+淋巴细胞和CD4^+CD25^+FOXP3^+T细胞增加  

Increased expressions of peripheral PD-1^+ lymphocytes and CD4^+CD25^+FOXP3^+ T cells in gastric adenocarcinoma patients

作　　者：李浩[1,2] 李松岩[2] 胡时栋[2] 邹贵军[2] 胡子龙[2] 魏华华[3] 王玉锋[4] 杜晓辉[2] 

机构地区：[1]中国人民解放军空军总医院招飞体检队,北京100142 [2]中国人民解放军总医院普通外科,北京100853 [3]北京大学国际医院护理部,北京102206 [4]中国人民解放军总医院住院管理科,北京100853

出　　处：《细胞与分子免疫学杂志》2017年第1期81-84,88,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(61471397)

摘　　要：目的检测胃腺癌患者外周血程序性死亡蛋白1(PD-1)阳性淋巴细胞和CD4^+CD25^+FOXP3^+T细胞的频数。方法选取胃腺癌术前患者29例,年龄、性别匹配的29例健康体检中心健康志愿者作为对照,流式细胞术检测两组外周血不同亚群淋巴细胞表面PD-1表达及CD4^+CD25^+FOXP3^+T细胞的频数。结果胃腺癌患者外周血淋巴细胞表面PD-1阳性率和CD4^+CD25^+FOXP3^+T细胞频数高于健康志愿者。胃腺癌患者外周血PD-1与CD4^+CD25^+FOXP3^+T细胞存在正相关。结论胃腺癌患者外周血PD-1^+淋巴细胞和CD4^+CD25^+FOXP3^+T细胞增加。Objective To detect the frequencies of peripheral programmed death-1 -＋（ PD-1 -＋） lymphocytes and CD4 -＋CD25-＋FOXP3-＋regulatory T cells in patients with gastric adenocarcinoma. Methods The study enrolled 29 patients with gastric adenocarcinoma and 29 age-and sex-matched healthy controls. Frequencies of PD-1-＋lymphocytes and CD4-＋CD25-＋FOXP3-＋regulatory T cells were detected using flow cytometry. Results The number of PD-1-＋lymphocytes and CD4-＋CD25-＋FOXP3-＋regulatory T cells in peripheral blood was higher in patients with gastric adenocarcinoma than that in the control group. Moreover,linear correlation analysis indicated a positive correlation between PD-1 expression and frequency of CD4-＋CD25-＋FOXP3-＋regulatory T cells in peripheral blood of the patients. Conclusion Gastric adenocarcinoma patients present with increased PD-1-＋lymphocytes and CD4-＋CD25-＋FOXP3-＋regulatory T cells in the peripheral blood.

关 键 词：胃癌 程序性死亡蛋白1(PD-1) 外周血 淋巴细胞 CD4+CD25+FOXP3+T细胞(调节性T细胞) 

分 类 号：R392.12[医药卫生—免疫学]