高原低氧暴露小鼠脾脏T淋巴细胞数量减少且免疫活性下降  

Decreased number and immune activity of splenic T lymphocytes in mice exposed to hypoxia at high altitude

作　　者：张晓娜[1] 李积东[1] 胡方杰[1] 高世兰 蒲小燕[1] 永胜[1] ZHANG Xiaona LI Jidong HU Fangjie GAO Shilan PU Xiaoyan YONG Sheng(Medical College, Qinghai University, Xining 810001, China)

机构地区：[1]青海大学医学院,青海西宁810001

出　　处：《细胞与分子免疫学杂志》2017年第2期164-167,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81060249);青海省应用基础研究计划项目(2015-ZJ-741;2014-ZJ-715;2013-Z-905);青海大学2015年度中青年科研基金(2015-QYY-1)

摘　　要：目的研究高原低氧条件下,小鼠脾脏T淋巴细胞亚群及其功能的改变。方法分别在400 m、2200 m和4200 m的不同海拔条件下饲养小鼠30 d后,ELISA检测脾细胞培养上清中白细胞介素4(IL-4)、γ干扰素(IFN-γ)的含量;MTT法检测小鼠脾脏T淋巴细胞的增殖能力;流式细胞术观察小鼠脾脏T淋巴细胞亚群的变化。结果高海拔低氧暴露30 d后,与对照组(海拔400 m)相比,4200 m和2200 m实验组小鼠脾脏指数明显增高,4200 m低氧组小鼠脾脏指数与2200 m低氧组相比也显著增加;小鼠脾细胞培养上清中IFN-γ的含量明显降低,而IL-4的水平未发生明显变化;脾脏T淋巴细胞增殖能力明显降低,同时,4200 m低氧组与2200 m低氧组相比,其T细胞增殖能力也显著下降;脾脏CD3^+T细胞、CD4^+T细胞、CD8^+T细胞数显著下降,其中,CD4^+T细胞数较CD8^+T细胞数下降明显,另外,4200 m低氧组与2200 m低氧组相比,CD3^+T细胞、CD4^+T细胞数也明显减少。结论海拔4200 m和2200 m高原低氧暴露30 d后,小鼠脾脏T淋巴细胞数目减少且增殖能力降低,IFN-γ水平降低。Objective To explore the changes of splenic T lymphocyte subsets and functions in mice under high-altitude hypoxic conditions. Methods After mice were exposed to an altitude of 400 m,2200 m and 4200 m for 30 days,ELISA was used to detect the concentrations of interleukin-4（ IL-4） and interferon-γ（ IFN-γ） in the cultured splenocyte supernatant; MTT assay was used to analyze the proliferation of splenic T lymphocytes; flow cytometry was performed to examine the alterations of splenic T lymphocyte subsets. Results After exposed to hypoxia for 30 days,in comparison with the control group（ 400 m）,the spleen index of the mice increased significantly in both the 2200 m and 4200 m groups,and the spleen index of the 4200 m group was apparently higher than that of the 2200 m group; the concentration of IFN-γ in the splenocyte supernatant of the 4200 m and 2200 m groups significantly decreased,while the concentration of IL-4 had no obvious change. The proliferation of splenic T lymphocyte was reduced obviously in both the 2200 m and 4200 m groups,at the same time,the proliferation of T cells in the 4200 m group was markedly lower than that in the 2200 m group. The percentages of splenic CD3^＋,CD4^＋and CD8^＋T lymphocytes decreased markedly in the 2200 m and 4200 m groups,among them,the number of CD4^＋T cel s decreased significantly than CD8^＋T cel s. In addition,CD3^＋and CD4^＋T lymphocyte percentages of the 4200 m group obviously decreased compared with the 2200 m group. Conclusion In mice exposed to hypoxia at an altitude of 4200 m and 2200 m for 30 days,the number of T lymphocyte subsets and the proliferation ability of T cel s decrease,and the level of IFN-γ is decreased as wel.

关 键 词：高原低氧 T淋巴细胞 细胞因子 流式细胞术 

分 类 号：R392.12[医药卫生—免疫学] Q253[医药卫生—基础医学]