附子甘草的HPLC检测方法学研究  

Analyticalof Radix Aconiti Carmichaeli- Glycyrrhiza Uralensis by HPLC

作　　者：杜欣韵 刘宁芝 王惠[1] 蒋建兰[1] 

机构地区：[1]天津市生物与制药工程重点实验室,天津大学化工学院制药工程系,天津300354

出　　处：《现代化工》2017年第3期203-206,共4页Modern Chemical Industry

摘　　要：利用高效液相色谱(HPLC)建立了附子甘草水提物的检测方法,并进行方法学考察。利用Waters高效液相色谱仪对色谱柱、流动相组成、洗脱程序、检测波长、柱温、流速等因素进行考察,最终确定选用Waters Symmetry C18色谱柱,以5 mmol/L醋酸铵水溶液(含0.1%冰醋酸)-乙腈为流动相进行梯度洗脱,检测波长为254 nm,流速为0.4 m L/min,柱温为30℃;以氢溴酸高乌甲素为内标物,各色谱峰相对保留时间及相对峰面积为指标进行方法学考察,结果表明,该方法重复性、重现性、稳定性良好,RSD均在5%以内。A method for analysis of the components extracted from Radix Aconiti Carmichaeli-Glycyrrhiza Uralensis is established by HPLC. Using Waters e2695 HPLC,several factors have been studied,including chromatographic column,composition of mobile phase,elution program,detection wavelength,column temperature and flow rate.Chromatographic conditions are determined as follows： waters symmetry C18 as chromatographic column,a mixture of 5mmol / L ammonium acetate（ containing 0. 1% glacial acetic acid） and acetonitrile as mobile phase,254 nm of detection wavelength,0. 4 m L / min of flow rate,30℃ of column temperature and Lappaconitine as the internal standard substance.Both of the relative retention time and relative peak area are analyzed to make the study of methodological observation.The result proves that the established method is accurate,reproducible and stable. All the RSDs are ≤5%.

关 键 词：附子 甘草 HPLC 方法学 

分 类 号：R284.1[医药卫生—中药学]