利胆汤对大鼠急性重症胆管炎所致的肝损伤的作用机制  

Lidantang attenuates severe acute cholangitis induced hepatic injury

作　　者：王建[1] 陈卫东[1] 刘兴洲[1] 吴永丰[1] WANG Jian CHEN Weidong LIU Xingzhou WU Yongfeng(Zhongda Hospital, Southeast University （Jiangbei）, Nanjing 210009, China)

机构地区：[1]东南大学附属中大医院江北院区,南京210009

出　　处：《临床与病理杂志》2017年第2期353-358,共6页Journal of Clinical and Pathological Research

摘　　要：目的:研究利胆汤对急性重症胆管炎的大鼠所致肝损伤的作用机制。方法:选择健康的Wistar大鼠60只,3月龄,雄性,随机分组为对照组、模型组、胆宁片组、利胆汤低剂量组、中剂量组和高剂量组6组,每组10只。分别于术后立刻、术后6 h、术后12 h、术后24 h 4个时间点收集大鼠胆汁,以及检测大鼠胆总管压力;对照组和模型组灌胃给予0.9%的氯化钠;胆宁片组灌胃给予胆宁片0.5 g/(kg·d);各利胆汤剂量组:6,12和24 g/(kg·d),灌胃给药3天后处死大鼠。HE染色观察大鼠肝病理形态;ELISA检测的方法大鼠血清中的细胞因子IL-6,TNF-α水平;Q-PCR法检测大鼠肝脏巨噬细胞移动抑制因子(migration inhibitory factor,MIF)和Toll样受体4(Toll-like receptor 4,TLR4)基因的表达。结果:利胆汤能明显的降低血清细胞因子IL-6(P<0.05)、TNF-α的表达(P<0.01),并且明显抑制MIF和TLR4的表达(P<0.01)。结论:利胆汤能够改善大鼠急性重症胆管炎所致的肝损伤,作用机制可能通过抑制MIF和TLR4的表达和下调IL-6,TNF-α的表达水平实现的。Objective： To investigate the mechanism of lidantang for treatment of hepatic injury induced by severe acute cholangitis. Methods： Sixty male Wistar rats were randomly divided into Control group （0.9% NaCl）, Model group （0.9% NaCl）, Danningpian group （0.5 g/kg）, and lidantang group [6, 12, 24 g/（kg·d）]. At 0, 6, 12, 24 h post-surgery, the rat bile was collected, and the pressure of duct were measured. After 3 days of intragastric administration and all rats were killed, pathological morphologic change were observed by HE staining, ELISA was used to assess the levels of serum cytokines IL-6, TNF-α, macrophage migration inhibitory factor （MIF） and Toll-like receptor 4 （TLR4） was assessed by Q-PCR. Results： Lidantang could decrease the level of serum TNF-α （P〈0.01） and reduce the level of serum IL-6 （P〈0.05）, moreover, lidantang could also decrease the expression of MIF （P〈0.01） and TLR4 （P〈0.01）. Conclusion： Lidantang can attenuate severe acute cholangitis induced hepatic injury via down-regulate the serum of IL-6, TNF-α and inhibit the expression of MIF, TLR4 in the liver.

关 键 词：利胆汤 胆管炎 肿瘤坏死因子-α 白细胞介素-6 移动抑制因子 TOLL样受体4 

分 类 号：R285.5[医药卫生—中药学]