表观遗传试剂诱导海洋真菌Aspergillus versicolor DJ013产生次级代谢产物的研究  

Epigenetic manipulation regulates secondary metabolites from the marine-derived fungus Aspergillus versicolor DJ013

作　　者：朱静轩[1] 王婷婷[1] 周珂欣 丁立建[1] 吴小凯[1] 何山[1] 

机构地区：[1]宁波大学李达三海洋生物医药研究中心,浙江宁波315211

出　　处：《中国海洋药物》2017年第1期14-18,共5页Chinese Journal of Marine Drugs

基　　金：宁波市科技富民项目(2015C10026);宁波大学学科项目(XKl15D237)资助

摘　　要：目的对1株来源于浙江东极岛海域沉积物的海洋真菌Aspergillusversicolor进行表观遗传试剂2-hexyl-4-pentynoic acid诱导并研究其次级代谢产物。方法采用溶剂萃取、柱层析色谱及制备色谱等方法对化合物进行分离纯化,通过LC-MS和NMR等方法进行结构鉴定,采用滤纸片扩散法测定抗菌活性。结果在表观遗传试剂2-hexyl-4-pentynoic acid诱导下,从Aspergillus versicolor分离得到3个化合物,经结构鉴定为弯孢霉菌毒素(1)、cyclo-(L-Trp-L-Phe)(2)和diorcinol(3)。Objective To study the secondary metabolites of fungus Aspergillus versicolor regulated by epigenetie manipulation 2-hexyl-4-pentynoie acid from the Dong Ji island. Methods The extracts were purified by solvent extraction, column chromatography and preparative chromatography, to establish their structures with LC-MS and NMR, their biological activities were evaluated by filter paper disper- sion method. Results Three compounds were purified from Aspergillus versicolor regulated by 2-hexyl- 4-pentynoic acid, they were identified as eurvularin（1）, eyelo-（L-Trp-L-Phe） （2）, diorcinol （3）.

关 键 词：表观遗传修饰 2-hexyl-4-pentynoic ACID 海洋真菌 次级代谢产物 

分 类 号：R391[医药卫生—基础医学]