甲壳素温敏水凝胶止血作用的研究  

Study on hemostatic function of a chitin thermo-sensitive hydrogel

作　　者：陈全[1] 王慧[1] 石杰[1] 彭燕飞[1] 刘万顺[1] 韩宝芹[1] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《中国海洋药物》2017年第1期71-75,共5页Chinese Journal of Marine Drugs

基　　金：国家高技术研究发展计划项目(2014AA093605);山东省自主创新及成果转化专项(2014ZZCX06203)资助

摘　　要：目的评价甲壳素温敏水凝胶的止血作用。方法制作大鼠肝脏损伤出血模型,分为空白对照组、阳性对照组、实验组,空白对照组肝脏出血创面不使用止血材料,阳性对照组、实验组肝脏出血创面分别使用纤维蛋白黏合剂和甲壳素温敏水凝胶止血,记录出血时间和出血量,于术后测定血清中TGF-β1和IL-6的含量,观察肝脏愈合情况,对损伤肝脏进行组织学观察。结果在手术中使用甲壳素温敏水凝胶能够快速止血,大鼠术后血清中TGF-β1和IL-6含量适中,在愈合过程中炎症反应轻。Objective To evaluate the hemostatic function of a chitin thermo-sensitive hydrogel. Methods The solution of thermo-sensitive chitin was injected on the surface of rats bleeding liver, both of bleeding time and blood loss were recorded. Fibrin glue was used as positive control. None of hemostatic agents were taken in blank control. Gross observation and histological analysis were taken to evaluate the condition of liver healing, and concentration of TGF-β1 and IL-6 were determined by Elisa assay kit in post operation. Results Quick hemostasis could be achieved by using the chitin thermo-sensitive hy- drogel in surgery, modest concentrations of TGF-β1 and IL-6 were determined, and slight inflammation was observed after operation.

关 键 词：甲壳素 凝胶 止血 

分 类 号：R965[医药卫生—药理学]