转录因子二聚化配体3(TFDP3)在乳腺癌组织中的表达及其与乳腺癌分子分型的相关性研究  

The Expression of TFDP3 in Breast Cancer and Its Role of Breast Cancer Molecular Classification

作　　者：陈雪[1] 初明[1] 杨珂[1] 阴凯麟 何嘉勰[1] 徐兰[1] 焦运燊 丁羚昱 刘彦辰[2] 初正云[2] 王月丹[1] 

机构地区：[1]北京大学医学部基础医学院,北京100191 [2]辽宁中医药大学药学院,辽宁沈阳110032

出　　处：《现代生物医学进展》2017年第1期1-6,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81172884);北京市自然科学基金项目(7162099);国家基础科学人才培养基金项目(J1030831/J0108);深圳市2015年科技研发资金基础研究项目;北京大学创新人才培养计划;北京大学医学部新教师启动基金项目(BMU0239)

摘　　要：目的:本研究旨在分析总结癌-睾丸抗原TFDP3在乳腺癌中的表达规律,探究TFDP3表达与乳腺癌分型及发病进程关系。方法:通过对不同分型及分期的乳腺癌组织切片进行免疫组化检测,分析TFDP3在其中的表达情况,并结合样本来源患者的临床信息,对TFDP3的表达与乳腺癌分子分型、分期及预后的相关性进行统计分析。同时,通过Western Blot检测了5种乳腺癌细胞系(MDA-MB-231、MCF-7、SK-BR-3、T47D及MCF-10A)中TFDP3的表达情况,并通过细胞免疫荧光实验,检测TFDP3在细胞中的定位。结果:TFDP3在已检测的乳腺癌样本中的表达率为49%。乳腺癌临床分子分型标记物HER2的表达与TFDP3的表达呈正相关,但乳腺癌的分期、临床病理分型以及临床分子分型标记物ER、PR的表达均与肿瘤组织中TFDP3的表达无相关性。结论:TFDP3在各在乳腺癌组织中高表达,其表达量与HER2的表达量呈正性相关。这为研究已TFDP3为靶点的乳腺癌免疫治疗,提供了新的依据。Objective： The object of this study is to analysis the expression of cancer testis antigen TFDP3 in breast cancer, to explore the correlation between TFDP3 and development of breast cancer. Methods： The expression of TFDP3 was detected in pathological section of tumor tissues from patients with breast cancer using immunohistochemical method. And we analyzed the data with clinical information of patients to discover the correlation of TFDP3 and the molecular classification of breast cancer. We also detected the expression of TFDP3 protein in five breast cancer cell lines, including MDA-MB-231, MCF-7, SK-BR-3, T47 D and MCF-10 A, using Western Blot. Then we verified the location of TFDP3 protein in cells through immunofluorescence method. Results： TFDP3 is greatly and broadly expressed in many cell lines and breast cancer tissues, such as breast, lymph node, hypothyroid, appendix and so on, while TFDP3 is 49%percent positive in breast cancer. The expression of TFDP3 is unrelated with clinical stage, pathology type and molecular classification of breast cancer, but related with special tumor marker HER2. Conclusion： TFDP3 highly expresses in breast cancer is related with the expression of HER2. It suggests that TFDP3 be a new potential target molecule of immunotherapy for breast cancer.

关 键 词：乳腺癌 转录因子二聚化配体3 癌睾丸抗原 HER-2 分子分型 

分 类 号：Q753[生物学—分子生物学]