侧脑室注射Orexins对大鼠摄食的影响及机制  

Effects of Orexins on the Food Intake of Rats and Its Mechanism

作　　者：张莉[1,2] 张孟玲[1] 郭菲菲[1] 孙向荣[1] 高胜利[1] 徐珞[1] 

机构地区：[1]青岛大学医学部基础学院病理生理教研室,山东青岛266021 [2]高密市人民医院麻醉科,山东高密261500

出　　处：《现代生物医学进展》2017年第1期28-31,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81470815;81270460;81300281;81500414);山东省优秀中青年科学家科研奖励基金项目(BS2014YY009);青岛市科技局基金项目(13-1-4-170-jch;14-2-3-3-nsh)

摘　　要：目的:探讨侧脑室注射orexins(食欲素)、NPY(神经肽Y)、MCH(黑色素聚集激素)和甘丙肽对大鼠摄食的影响及其机制。方法:将成年雄性Wistar大鼠随机分为对照组、侧脑室注射组和室旁核(PVN)注射组。通过套管将orexin-A、orexin-B、NPY、MCH和甘丙肽分别注射至侧脑室和PVN内,随后测量大鼠食物摄入量,并检测PVN、弓状核(ARC)和VMH内c-fos的表达。结果:与对照组比较,侧脑室注射NPY、MCH和orexin-B 2 h后,大鼠摄食量显著增多(P<0.05)。相较于orexin-B和MCH,NPY对摄食的影响更显著(P<0.05)。与NS对照组比较,侧脑室注射甘丙肽和orexin-A 1 h后,大鼠摄食量显著增多(P<0.05)。与NS对照组比较,侧脑室注射orexin-A可显著增加c-fos在PVN和ARC中的表达,在VMH中效应较弱(P<0.05)。与NS对照组比较,PVN注射NPY能显著增加大鼠2 h摄食量(P<0.05),PVN注射orexin-A能显著增加大鼠2 h和4 h摄食量(P<0.05)。结论:orexins与可促进大鼠摄食,此效应可能通过下丘脑参与摄食调控中枢PVN和ARC而实现的。Objective： To investigate the role of the orexins, NPY, melanin-concentrating hormone（MCH） and galanin in food intake in rats and their mechanism. Methods： Male wistar rats were randomly divided into sham group, lateral ventricle（ICV） group and PVN group. Orexin-A, orexin-B, NPY, MCH and galanin were respectively injected into ICV and PVN through the cannula, then measured the food intake of rats to compared their effect. Also the expression of c-fos was detected in PVN, ARC and VMH. Results： Compared with sham group, the 2 h food intake of rats following injected NPY, MCH and orexin-B into ICV had significantly increased（P〈0.05）. The effect of NPY in food intake was more obviously than orexin-B and MCH（P〈0.05）. Compared with sham group, the 1 h food intake of rats following inject galanin and orexin-A into ICV had significantly increased（P〈0.05）. C-fos was expressed in PVN and ARC markedly（P〈0.05）. Compared with sham group, the expression of c-fos was greatly inhanced after orexin-A administered into ICV, weakly in VMH（P〈0.05）. Compared with sham group, the 2 h food intake of rats following injected NPY into PVN had significantly increased（P〈0.05）, orexin-A markedly increased the food intake of rats at 2 h and 4 h（P〈0.05）. Conclusions： Orexin-A can stimulated food intake of rats, and it may related to its physiology role in appetite regulation.

关 键 词：OREXINS NPY MCH 甘丙肽 摄食 

分 类 号：R-33[医药卫生]