模拟微重力通过调节AKT磷酸化抑制前成骨细胞的增殖  

Simulated Microgravity Inhibits the Proliferation of Osteoblasts by Regulating the Phosphorylation of AKT

作　　者：周骅[1] 曹新生[1] 胡泽兵[1] 王瀚[1] 王艺璇[1] 张舒[1] 

机构地区：[1]第四军医大学航空航天生物动力学教研室,陕西西安710032

出　　处：《现代生物医学进展》2017年第2期229-232,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(31570939;81471815;31170889)

摘　　要：目的:研究模拟微重力对前成骨细胞的增殖作用及其分子机制。方法:利用2D回转器模拟失重条件培养前成骨细胞MC3T3-E1 24小时;将p-AKT激活剂SC79加入细胞培养基后模拟失重条件下培养前成骨细胞MC3T3-E1 24小时;利用Western blot技术分别检测细胞增殖相关蛋白PCNA以及AKT、p-AKT的表达变化情况。结果:(1)与对照组相比,模拟失重组前成骨细胞增殖受到抑制(P<0.01),p-AKT/AKT比值减小(P<0.01);(2)加入SC79组与对照组相比,p-AKT/AKT比值显著增加;(3)加入SC79的模拟失重组(MG+SC79)与模拟失重组相比(MG),p-AKT/AKT比值增加,PCNA蛋白表达增加(P<0.01),成骨细胞增殖有所恢复。结论:模拟微重力可能通过抑制AKT的磷酸化形式抑制前成骨细胞的增殖,加入p-AKT激活剂可部分恢复前成骨细胞的增殖。Objective： To investigate the effect of simulated microgravity on the proliferation and the molecular mechanism of the osteoblast. Methods： 2D rotator was used to simulate weightlessness condition. The MC3T3-E1 cells were divided into 4 groups： control group（CON）, the simulated weightlessness group（MG）, the control with SC79 group（CON＋SC79） and simulated weightlessness treatment with SC79 group（MG＋SC79）. AKT phosphorylation activator SC79 was added into the cell culture medium before exposing to simulated weightlessness, then all the MC3T3-E1 cells were cultured for 24 h. Changes among MC3T3-E1 cells were detected by Western blot analysis, which including cell proliferation related protein PCNA, p-AKT and AKT. Results：① Compared with the CON group, cell proliferation of MG group is inhibited（P〈0.01）, and the ratio of p-AKT/AKT was decreased（P〈0.01）.②Compared with the CON group, the ratio of p-AKT/AKT of CON＋SC79 group was raised.③Compared with MG group, p-AKT / AKT ratio was increased,PCNA protein expression was increased partially（P〈0.01）, and osteoblast proliferation had been restored in the MG＋SC79 group.Conclusion： Simulated microgravity can inhibit the proliferation of osteoblasts by inhibiting the phosphorylation of AKT. The proliferation of osteoblasts are partially restored by the addition of p-AKT activator.

关 键 词：模拟微重力 MC3T3-E1细胞 AKT 增殖 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]