石甘散散剂挥发油的提取工艺研究  

Extraction Technology Research on Volatile Oil of Shi Gan Powder

作　　者：李兵[1] 王亮亮[1] 张丽杰[1] 李龙[1] 程为平[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《现代生物医学进展》2017年第2期233-236,246,共5页Progress in Modern Biomedicine

基　　金：黑龙江省自然科学基金项目(051478)

摘　　要：目的:探讨石甘散散剂挥发油提取工艺,优选石甘散散剂挥发油提取方法,确定提取的最佳工艺条件。方法:利用正交试验法,以水蒸气蒸馏法提取石甘散挥发油,计算挥发油得率;并用高效液相色谱法,以面积归一化法计算石甘散挥发油中β-细辛醚的含量为指标,研究石甘散挥发油提取工艺中的影响因素;对提取时间、浸泡时间和加水量三个影响因素的试验结果进行分析,计算不同因素影响下的石甘散散剂挥发油得率及β-细辛醚的含量,从而优选出石甘散挥发油最佳提取工艺。结果:影响石甘散挥发油得率及β-细辛醚含量的最主要因素为提取时间,其次为加水量,再者为浸泡时间。结论:通过这三个因素影响下的正交试验结果,筛选出的石甘散散剂挥发油最佳提取工艺为加水量10倍,浸泡时间1小时,提取时间8小时。该提取工艺简单易行、合理稳定,为石甘散散剂挥发油的最佳提取工艺条件。Objective： Discussing the extraction process of volatile oil from Shi Gan powder, optimizing the extraction method of it, to determine the optimal extraction process. Methods： Using orthogonal test method, with volatile oil extracted by steam distillation, to calculate the rate of volatile oil. Using high performance liquid chromatography to calculate the content of β-asarone from volatile oil of Shi Gan powder, with Area normalization as calculating method. To study the influence factors in the extraction of Shi Gan powder and analyse the results of the experiment from the aspects of extraction time, soaking time and water amount. Calculating the rate of volatile oil and the content of β-asarone from volatile oil of Shi Gan powder under the influence of the different factors, and thereby to optimize the extraction process of volatile oil from Shi Gan powder. Results： Extraction time was the first influence factor of the rate of extraction and the content of β-asarone, and the second is the amount of water, and the third is the soaking time. Conclusion： Through orthogonal test under these three influence factors, screening out the optimal extraction process of volatile oil from Shi Gan powder was 10 times the amount of water, soaking time for 1 hour and extraction time for 8 hours. The extraction process was simple, reasonable and stable, and the optimum extraction condition of Shi Gan powder was determined.

关 键 词：提取工艺 正交试验 石甘散散剂 挥发油 Β-细辛醚 

分 类 号：R284[医药卫生—中药学] R286[医药卫生—中医学]