雌激素受体单核苷酸多态性与子宫颈癌的相关性研究  

Association of estrogen receptor single nucleotide polymorphism with cervical cancer

作　　者：陈莉婷[1] 彭敏[1] 莫菊玲[1] 

机构地区：[1]佛山市妇幼保健院妇科,广东佛山528000

出　　处：《中国妇幼健康研究》2017年第2期145-148,共4页Chinese Journal of Woman and Child Health Research

摘　　要：目的探讨雌激素受体(GPR30)单核苷酸多态性与子宫颈癌的相关性。方法选取114例子宫颈癌患者为研究组,同数量子宫息肉及子宫肌瘤等良性疾病者为对照组,采用S-P免疫组化法测定子宫颈癌组织GPR30表达,PCR-RFLP法测定GPR30(rs3808351)位点基因多态性。结果对照组与研究组在怀孕次数、首次性生活年龄、文化程度及HPV感染方面有统计学差异(χ~2=20.64、14.05、10.11、39.71,P<0.05);研究组GPR30阳性表达率显著高于对照组(χ~2=12.65,P<0.05);对照组与研究组GG、AA基因型比较差异有统计学意义(χ~2值分别为6.14、5.89,均P<0.05),G、A等位基因频率比较差异有统计学意义(χ~2值分别为5.63和5.76,均P<0.05);Logistic回归分析显示,怀孕次数(OR=1.659,95%CI:1.231~4.964,P<0.05)、HPV感染(OR=1.778,95%CI:1.196~3.854,P<0.05)、G等位基因频率(OR=2.239,95%CI:1.545~7.032,P<0.05)为子宫颈癌的危险因素。结论 GPR30(rs2808350)位点G等位基因频率可能为子宫颈癌的发生危险因素。Objective To investigate the association of single nucleotide polymorphism of estrogen receptor （GPR30） and cervical cancer. Methods Totally 114 cases of cervical cancer were selected in study group, while the same number of cases of uterine polyps, uterine fibroids and other benign diseases were in control group. GPR30 expression in cervical cancer tissues was determined by S-P immunohistoehemical method, and PCR-RFLP method was used for the determination of GPR30 （ rs3808351 ） gene polymorphism. Results There were statistical differences in the times of pregnancies, the first sex age, education level and HPV infection between the study group and the control group （X2 value was 20.64, 14.05. 10. 11 and 39.71, respectively, all P 〈0.05）. The positive rate of GPR30 in the study group was significantly higher than that in the control group （X2 = 12.65, P 〈 0.05 ）. GG and AA genotypes were significantly different between the study group and the control group （X2 value was 6.14 and 5.89, respectively, both P 〈 0.05 ）, so were the allele frequencies of G and A （X2 value was 5.63 and 5.76, respectively, both P 〈 0.05 ）. Logistic regression analysis showed that the number of pregnancies （ OR = 1. 659, 95 % CI： 1.231 - 4. 964, P 〈 0.05 ）, HPV infection （ OR = 1. 778,95 % CI： 1. 196 - 3. 854, P 〈0. 05 ） and G allele （OR = 2.239, 95% CI： 1. 545 -7. 032, P 〈 0.05 ） were the risk factors for cervical cancer. Conclusion G allele frequency on GPR30 （rs2808350） locus may be the risk factors for cervical cancer.

关 键 词：G蛋白偶联受体30 子宫颈癌 单核苷酸多态性 基因 

分 类 号：R711.74[医药卫生—妇产科学]