高效液相色谱法测定人参提取物中的苯并(α)芘  

Determination of Benzo(α) Pyrene in Ginseng Extract via High Performance Liquid Chromatography

作　　者：田芳洁[1] 康明芹[1] 曹海微[1] 李荣荣[1] 刘泽朋[1] 陈明岩[1] TIAN Fang-fie KANG Ming-qin CAO Hai-wei Ll Rong-rong LIU Ze-peng CHEN Ming-yan(Jilin Entry-Exit Inspection and Quarantine Bureau, Changchun 130062, China)

机构地区：[1]吉林出入境检验检疫局,吉林长春130062

出　　处：《化学试剂》2017年第3期266-268,280,共4页Chemical Reagents

基　　金：国家质检总局科技资助项目(2012IK162;2013-IK162)

摘　　要：建立了人参提取物中苯并(α)芘残留量的高效液相色谱分析方法。样品加水浸泡,用正己烷液液萃取,提取液减压蒸干,C18色谱柱分离,以乙腈-水为流动相,外标法定量。结果表明,苯并(α)芘在1.0~100.0μg/L范围内线性良好,相关系数R为0.999 9,平均加标回收率为85.2%~96.7%,相对标准偏差为7.1%~11.2%。方法的定量限为1.0μg/kg。该方法简单快速、灵敏度高、重现性好,能满足人参提取物中苯并(α)芘残留量定量分析的要求。A method for the determination of benzo（α） pyrene in ginseng extract was developed using high performance liquid chromatography.The samples were soaked with water,extracted with n-hexane,evaporated in vacuum,separated by C18 column with acetonitrile and water as mobile phase and quantitative by external standard method.The results showed that benzo（α） pyrene had a good linear relationship in the range of 1.0-100.0 μg/L and the relative coefficient was 0.999 9.The spiked recoveries was 85.2%-96.7% and the relative standard deviations was 7.1%-11.2%.The limit of quantitation of the method was1.0 μg/kg.The simple and rapid method with good reproducibility can be used for the determination of benzo（α） pyrene in ginseng extract.

关 键 词：液液萃取 高效液相色谱 苯并(α)芘 人参提取物 

分 类 号：O657.72[理学—分析化学]