汝州白三叶草根瘤菌的分离与分子鉴定  

The isolation and molecular identification of Rhizobia associated with white clover from Ruzhou

作　　者：张俊杰[1] 杨旭[1] 陆勇[1] 张志炎[1] 王志伟[1] 魏喜旺[1] ZHANG Junjie YANG Xu LU Yong ZHANG Zhiyan WANG Zhiwei WEI Xiwang(College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, Chin)

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450002

出　　处：《河南农业大学学报》2017年第1期76-81,共6页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(31400008);郑州轻工业学院博士科研基金项目(2014bsjj006)

摘　　要：本研究采用16S r DNA PCR-RFLP、IGS PCR-RFLP和16S r DNA基因测序和系统发育分析等分子鉴定方法,对分离的汝州白三叶草共生根瘤菌进行了多样性研究。结果表明,21株供试菌株仅产生了一种16S r DNA酶切图谱组合类型,通过IGS PCR-RFLP又把所有的供试菌株分为了6种酶切图谱类型。经过对代表菌株的16S r DNA基因测序及系统发育分析,6个代表菌株之间具有99.9%~100%的相似性,且供试菌株与根瘤菌属的已知种群Rhizobium anhuiense和Rhizobium leguminosarum等也具有99.9%~100%的相似性,因此,分离自汝州的白三叶草根瘤菌属于根瘤菌属(Rhizobium)。A series of molecular identification methods including 16 S r DNA PCR-RFLP（ Restriction Fragment Length Polymorphism）,IGS PCR-RFLP,16 S r DNA gene sequencing and phylogenetic analysis were used to study the genetic diversity of clover rhizobia from Ruzhou. The results showed that a total of 21 clover rhizobia were obtained and they were of the same kind of 16 S r DNA PCR-RFLP pattern and were divided into six IGS PCR-RFLP patterns. Through the 16 S r DNA gene sequencing and phylogenetic alalysis of the representative strains,the representatives were with inner group similarities of 99. 9-100% and were with 99. 9% ~ 100% similarities of the known Rhizobium spp.,such as R.anhuiense and R. leguminosarum. So the clover rhizobia isolated from Ruzhou can be identified as Rhizobium strains.

关 键 词：白三叶草 根瘤菌 16S RDNA IGS RFLP 

分 类 号：Q939.114[生物学—微生物学]