大肠杆菌外膜蛋白OmpC的生物信息学分析及表位多肽疫苗的重组预测  

Bioinformatic analysis of E. coli OmpC and recombinant prediction of epitope peptide vaccine

作　　者：俱雄 党亚锋[1,3] 刘祥[1,2] 丁锐[1,2] 陈琛[1,2] 冯自立[1,2] 张辰露[1,2] JU Xiong DANG Yafeng LIU Xiang DING Rui CHEN Chen FENG Zili ZHANG Chenlu(Chinese-German Joint Institute for Natural Product Research, Shanxi Sci-Tech University, Hanzhong 723001, China The Food and Drug Inspection and Testing Center of Hanzhong City, Hanzhong 723000, China Shanxi Engineering Research Center for Tall Gastrodia Tuber and Medical Dogwood, Hanzhong 723001, China)

机构地区：[1]陕西理工大学中德天然产物研究所,陕西汉中723001 [2]陕西省天麻山茱萸工程技术研究中心,陕西汉中723001 [3]汉中市食品药品检验检测中心,陕西汉中723000

出　　处：《河南农业大学学报》2017年第1期94-100,共7页Journal of Henan Agricultural University

基　　金：陕西省农业科技创新与攻关项目(2016NY-088);陕西理工大学校级科研项目(SLGKY16-13)

摘　　要：为设计大肠杆菌外膜蛋白OmpC的表位多肽重组疫苗,进一步提高OmpC蛋白的免疫效果,通过生物信息学软件对OmpC蛋白的进化关系、高级结构与细胞表位进行分析。结果表明,大肠杆菌、沙门伤寒菌、肺炎克雷伯菌,这3种细菌具有较高同源性,OmpC蛋白可能对这3种菌株的感染起到交叉免疫保护作用。OmpC为亲水性蛋白,1~21位氨基酸为信号肽位置,无跨膜结构并定位于细胞膜外;OmpC二级结构中含无规则卷曲43.05%,a-螺旋16.08%,β-转角0%,β-片层40.87%。OmpC蛋白可能存在5个优势B细胞表位,1个CTL表位,1个Th细胞表位;并重组拼接获得抗原性较好的OmpC蛋白表位多肽。In order to improve the immune effect of OmpC（ outer membrane protein C） protein,the recombinant vaccine of Escherichia coli OmpC epitope polypeptide was designed. By bioinformatic software,the evolution relationship,advanced structure and cell epitopes of OmpC protein were analyzed.The results showed that the OmpC protein sequences of Escherichia coli,Salmonella,Klebsiella pneumoniae,had high homology,and OmpC antibodies might play a cross immune protective effect for these strains. OmpC was a hydrophilic protein,1 ~ 21 amino acids of OmpC was signal peptide sequence,and had not transmembrane structure being located outside the cell membrane. The OmpC secondary structure contained 43. 05% of random coil,16. 08% of alpha helix,0% of beta turn,and 40. 87% of extended strand,respectively. OmpC might have 5 B-cell epitopes,1 CTL epitopes,and 1 Th epitopes,and OmpC protein epitope vaccine with good immunogenicity was obtained by recombinant splicing.

关 键 词：大肠杆菌 OmpC蛋白 表位疫苗 抗原分析 

分 类 号：Q816[生物学—生物工程]