胡萝卜根中主要类胡萝卜素含量相关QTL的精细定位  

Fine Mapping of QTLs Related to Main Carotenoids in Carrot Root

作　　者：欧承刚[1] 孙婷婷[1] 刘新艳[1] 李成江 徐东辉[1] 赵志伟[1] 庄飞云[1] 

机构地区：[1]中国农业科学院蔬菜花卉研究所农业部园艺作物生物学与种质创制重点实验室,北京100081 [2]宿州农业科学院,安徽宿州234000

出　　处：《园艺学报》2017年第2期288-296,共9页Acta Horticulturae Sinica

基　　金：国家重点研发计划项目(2016YFD0100204-04);国家科技支撑计划项目(2013BAD01B04);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS);国家自然科学基金项目(31272162)

摘　　要：以白色的野生胡萝卜‘松滋野生’(Ws)和橘色的栽培胡萝卜品种‘Amsterdam’(Af)为亲本构建的回交重组自交系(BIL)为试材,基于低倍重测序技术开发SNP标记,构建了由1 976个Bin标记组成,包含29 435个SNP标记的遗传图谱。图谱总距离834.28 c M,平均图距0.42 c M。通过对胡萝卜肉质根中类胡萝卜素含量相关QTL分析,在连锁群LG04和LG08中检测到调控α–胡萝卜素、β–胡萝卜素、ζ–胡萝卜素、叶黄素、玉米黄质和总类胡萝卜素含量的主效QTL(M-QTL)2、2、3、2、2和2个,表型贡献率为11.47%~19.18%;另检测到调控α–胡萝卜素、β–胡萝卜素、ζ–胡萝卜素、玉米黄质和总类胡萝卜素含量的上位性QTL(E-QTL)1、1、2、1和1个,表型贡献率为2.50%~3.66%。在M-QTL显著区间内共检索到36个有功能注释的预测基因,其中Dck018297为ζ–胡萝卜素脱氢酶2基因,与调控β–胡萝卜素合成和总类胡萝卜素含量有关;Dck008006为乙烯响应因子2.2的同源基因,与调控α–胡萝卜素、ζ–胡萝卜素合成有关;Dck029898为转录因子b HLH135的同源基因,与调控玉米黄质合成有关。Based on whole-genome resequencing data,a high resolution genetic map was developed by using a set of backcross inbred lines（BILs）crossed by a wild species‘Songzi’with a cultivar‘Amsterdam’,and used to map QTLs for main carotenoids in carrot root. Finally,the genetic map was consisted of 1 976 Bins which contained 29 435 SNPs. The total genetic distance of map was 834.28 c M with an average interval of 0.42 c M between Bin markers. There were 2,2,3,2,2 and 2 main-effect QTLs（M-QTLs）associated with α-carotene,β-carotene,ζ-carotene,lutein,zeaxanthin and total carotenoid contents,respectively,with 11.47%–19.18% phenotypic variance. There were 1,1,2,1 and 1 epistatic QTLs contributed to α-carotene,β-carotene,ζ-carotene,zeaxanthin and total carotenoid contents,respectively,with 2.50%–3.66% phenotypic variance. A total of 36 predicted genes with function annotations were detected in M-QTLs. Among these,gene Dck018297 was ζ-carotene desaturase and associated with β-carotene biosynthesis and total carotenoid contents;gene Dck008006 homologouse to ERF2.2 was associated with α-carotene and zeaxanthin biosynthesis;gene Dck029898 homologouse to b HLH135 was associated with ζ-carotene biosynthesis. These three candidate genes might play central roles in the carotenoid biosynthesis in carrot root.

关 键 词：胡萝卜 类胡萝卜素 遗传图谱 精细定位 QTL 

分 类 号：S631.2[农业科学—蔬菜学]