HPLC法同时测定银黄颗粒中6种成分  

Simultaneous determination of six constituents in Yinhuang Granules by HPLC

作　　者：艾光丽 王维[1] 曾桢 蒲旭峰 AI Guang-li WANG Wei ZENG Zhen PU Xu-feng(College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611130, China Chengdu Municipal Institute for Food and Drug Control, Chengdu 610000, China)

机构地区：[1]成都中医药大学药学院,四川成都611130 [2]成都市食品药品检验研究院,四川成都610000

出　　处：《中成药》2017年第3期528-532,共5页Chinese Traditional Patent Medicine

基　　金：2015年成都市食品安全风险研究项目(2015cdfx01)

摘　　要：目的建立HPLC法同时测定银黄颗粒(金银花、黄芩提取物)中绿原酸、咖啡酸、木犀草苷、黄芩苷、木犀草素、芦丁的含有量。方法该药物80%甲醇提取液的分析采用Sun Fire-C18色谱柱(250 mm×4.6 mm,5μm);以乙腈-0.4%磷酸为流动相,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长239 nm。结果 6种成分在各自线性范围均呈良好的线性关系(r=0.999 9),平均加样回收率94.56%~98.38%,RSD 0.49%~2.89%。14批样品中黄芩苷含有量差异较小,其他5种成分含有量差异较大。结论不同厂家银黄颗粒和金银花提取物的质量参差不齐,应加强监管。AIM To establish an HPLC method for the simultaneous content determination of chlorogenic acid,caffeic acid,luteoloside,baicalein,luteolin and rutin in Yinhuang Granules（Lonicerae japonicae Flos and Scutellariae Radix extracts）.METHODS The analysis of 80% methanol extract of this drug was performed on a 30 ℃thermostatic Sun Fire-C18column（250 mm×4.6 mm,5 μm）,with the mobile phase comprising of acetonitrile-0.4% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at239 nm.RESULTS All the six constituents showed good linear relationships within their own ranges（r=0.999 9）,whose average recoveries were 94.56%-98.38% with the RSDs of 0.49%-2.89%.Among fourteen batches of samples,the luteoloside content was found to be of little difference,while the other five constituents’ contents were of relatively great differences.CONCLUSION The qualities of Yinhuang Granules and Lonicerae japonicae Flos extracts from different manufacturers are uneven,so supervision should be strengthened.

关 键 词：银黄颗粒 绿原酸 咖啡酸 木犀草苷 黄芩苷 木犀草素 芦丁 HPLC 

分 类 号：R927.2[医药卫生—药学]