C_(18)柱串联冠醚手性柱高效分离3种芳香族氨基酸对映体  

Enantiomeric Separation of Three Kinds of Aromatic Amino Acids by C_(18) Column Tandem Crown Ether Chiral Column

作　　者：刘育坚 刘智敏[1] 许志刚[1] 

机构地区：[1]昆明理工大学理学院,昆明650500

出　　处：《分析化学》2017年第3期369-373,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.21565018);昆明理工大学校级学科方向团队项目(No.14078356)资助~~

摘　　要：将C_(18)柱与手性冠醚柱串联,建立了一种反相高效液相色谱法用于3种芳香族氨基酸对映体同时拆分的方法。考察了反相色谱流动相的组成、p H值、柱温、流速对对映体拆分的影响。实验结果表明,当流动相为HCl O_4-乙腈溶液(86∶14,V/V,p H 2.0)、柱温20℃、流速0.4 m L/min时,3种氨基酸对映体可获得基线分离。进一步对比了C_(18)柱、冠醚手性柱和串联顺序不同的4种分离模式,结果表明,C_(18)柱不能拆分氨基酸对映体,仅能分离不同种类氨基酸;冠醚手性柱可分离氨基酸映体,但不同种类氨基酸色谱峰出现重叠;串联模式能实现3种氨基酸对映体的基线分离,实现双柱优势互补,而串联顺序对分离影响不大,仅影响色谱峰的峰形。A reversed phase high performance liquid chromatographic （RP-HPLC） method was proposed by using Cls column tandem crown ether chiral column for simultaneous separation of three kinds of aromatic amino acid enantiomers. The chromatographic conditions, including mobile phase proportion, pH, column temperature and flow rate, were optimized. The experimental results showed that the three aromatic amino acid enantiomers could be successfully separated under the optimal conditions such as perchloric acid solution- acetonitrile （86：14, V/V, pH = 2） as mobile phase, column temperature of 20℃ and flow rate of 0.4 mL/min. The separation under four HPLC column connection modes was further investigated. The results revealed that different kinds of amino acids could be separated on Cts column, but not for amino acid enantiomers; amino acid enantiomers could be separated on crown ether chiral column, but the chromatographic peaks of each amino acid enantiomers overlapped seriously; baseline separation of three amino acid enantiomers could be obtained under the connection of two columns, but the separation under different column connection sequence almost had no difference except the peak shape.

关 键 词：手性分离 芳香族氨基酸 对映体 冠醚柱 

分 类 号：O652.63[理学—分析化学]