泥浸汁对太湖沉积物中的好氧可培养细菌多样性的影响  

Effect of sediment extract on the culturable aerobic bacterial diversity in the sediments of Taihu Lake

作　　者：李晓丹[1] 屈建航[1] 周佳[1] 张璐洁[1] 李海峰[1] 田海龙[1] 

机构地区：[1]河南工业大学生物工程学院,河南郑州450000

出　　处：《微生物学通报》2017年第3期554-560,共7页Microbiology China

基　　金：国家自然科学基金项目(No.31370147;31400103);河南省高校科技创新团队支持计划(No.15IRTSTHN019);河南省属高校基本科研业务费专项资金(No.2014YWQQ16)~~

摘　　要：【目的】研究添加泥浸汁与否对太湖沉积物中可培养细菌的影响。【方法】采用R2A培养基和添加泥浸汁R2A培养基对沉积物中细菌进行分离培养,16S r RNA基因系统发育分析比较种群结构。【结果】培养基中添加泥浸汁,可使可培养细菌的种类数量增加到1.6倍。16S r RNA基因序列分析表明,培养的优势细菌类群存在明显差别。R2A培养基上生长的细菌主要为厚壁菌门(52%)、放线菌门(24%)、变形菌门(20%)和拟杆菌门(4%),其中大部分细菌与芽孢杆菌属、假单胞菌属、节杆菌属等关系密切;而添加泥浸汁的R2A培养基上生长的细菌则主要为变形菌门(40%)、放线菌门(35%)、厚壁菌门(22.5%)和拟杆菌门(2.5%),与鞘脂单胞菌属、芽孢杆菌属、副球菌属、节杆菌属等关系密切。【结论】添加泥浸汁原始营养因子可提高沉积物中可培养细菌的多样性,提高菌种可培养效率。[Objective] The study is aimed to research the effect of adding sediment extract or not on the cultivable bacterial diversity in the sediment of Taihu Lake. [Methods] By using R2 A medium and sediment extract R2 A medium to isolate and culture the bacteria in the sediment. Phylogenetic analysis based on the 16 S r RNA gene was used to identify the bacterial diversity. [Results] Results showed that the amount of the bacteria cultured on sediment extract R2 A increased up to 1.6 times more than that of on R2 A medium. Analysis based on the similarities of 16 S r RNA gene sequences of isolates presented effects on the dominant bacteria. On the R2 A medium, the dominant bacterial groups were Firmicutes（52%）, Actinobacteria（24%）, Proteobacteria（20%） and Bacteroidetes（4%）, and most of the bacteria isolated from R2 A were closely related to genera Bacillus, Pseudomonas and Archrobacter. However, the dominant bacterial groups isolated from sediment extract R2 A were Proteobacteria（40%）, Actinobacteria（35%）, Firmicutes（22.5%）, and Bacteroidetes（2.5%）. They are closely related to Sphingomonas, Bacillus, Paracoccus and Arthrobacter. [Conclusion] Adding the original nutrient factors of the sediment extract could increase the diversity of bacteria in the sediment and help to increase the cultivable efficiency.

关 键 词：可培养细菌 泥浸汁 沉积物 太湖 R2A培养基 

分 类 号：Q938.8[生物学—微生物学]