碳源对活性污泥微生物细胞膜特性和群落结构影响  

Effects of carbon sources on cell membrane properties and microbial community of activated sludge

作　　者：马思佳[1] 顾卓江 丁丽丽[1] 任洪强[1] 

机构地区：[1]污染控制与资源化研究国家重点实验室南京大学环境学院,江苏南京210023 [2]海正药业(杭州)有限公司,浙江杭州311404

出　　处：《微生物学通报》2017年第3期561-573,共13页Microbiology China

基　　金：国家高技术研究发展计划项目(863计划)(No.2012AA063407);国家科技支撑计划项目(No.2014BAC08B04)~~

摘　　要：【目的】揭示碳源对活性污泥微生物细胞膜特性(磷脂脂肪酸组成和流动性)以及群落结构的影响规律。【方法】采用原子力显微镜、磷脂脂肪酸(PLFA)、荧光漂白恢复(FRAP)和Mi Seq分析技术,考察以葡萄糖、乙酸钠、蛋白胨、葡萄糖?蛋白胨(1?1)和乙酸钠?蛋白胨(1?1)为基质的序批式活性污泥(SBR)反应器中,活性污泥微生物表面粘附力、细胞膜PLFA组成和流动性及群落结构的差异。【结果】含有蛋白胨为碳源时相比于葡萄糖和乙酸钠为单一碳源,细胞膜磷脂流动性增加,且18?1ω9c、15?0iso和17?0iso的含量提高了53.1%-354.7%、135.6%-407.9%和88.1%-264.3%;同时其微生物表面粘附力和群落多样性均增大。优势菌群对碳源的响应呈现出不一致的规律:葡萄糖和乙酸钠为单一碳源时其优势菌门分别为放线菌门和变形菌门,Nakamurella和Flavobacterium分别为其优势菌属,群落多样性指数分别为3.65和4.25;蛋白胨为碳源时促进了Candidatus Saccharibacteria门的累积,群落多样性指数在4.96-5.09。【结论】主成分分析(PCA)表明,含有蛋白胨为碳源时微生物细胞膜PLFA组成具有相似性;冗余分析(RDA)表明,不同碳源驯化出不同的微生物群落,进而也对细胞膜磷脂组成产生了影响。[Objective] The purpose of this study was to investigate the effects of carbon sources on cell membrane properties（phospholipid fatty acids composition and fluidity） and microbial community of activated sludge. [Methods] Atomic force microscopy（AFM）, phospholipid fatty acids（PLFA） and fluorescence recovery after photobleaching（FRAP）, and Mi Seq analysis technology were used to investigate the effects of the different substrates, glucose, sodium acetate, peptone, glucose？peptone（1？1）, sodium acetate？peptone（1？1）, on the differences of microbial surface adhesion force, PLFA composition and fluidity, and microbial community in five Sequencing Batch Reactors（SBRs）. [Results] The involvement of peptone led to an increase of cell membrane phospholipid fluidity, PLFA composition present a consistency with the content of 18？1ω9c, 15？0iso and 17？0iso increased 53.1%-354.7%, 135.6%-407.9% and 88.1%-264.3%, respectively. The dominant phylum response to carbon sources present inconsistent rules： with glucose and sodium acetate as solo substrate, the dominant phylum were Actinobacteria and Proteobacteria, and the dominant genus were Nakamurella and Flavobacterium, diversity index were 3.65 and 4.25, respectively. At the appearance of peptone, the content of Candidatus Saccharibacteria increased obviously, and the diversity index was in the range of 4.96-5.09. [Conclusion] Principal component analysis（PCA） indicated that the involvement of peptone led to similar PLFA composition. Redundancy analysis（RDA） suggested that carbon sources changed the microbial community, which further influenced the composition of PLFA.

关 键 词：活性污泥 碳源 磷脂脂肪酸(PLFA) 细胞膜磷脂流动性 微生物群落 

分 类 号：X703[环境科学与工程—环境工程] Q93[生物学—微生物学]