巴西橡胶树HbSERK1启动子的克隆及功能鉴定  

Molecular and Functional Characterization of the Hb SERK1 Promoter in Hevea brasiliensis

作　　者：王颖[1] 贾瑞[2] 李辉亮[1] 郭冬[1] 朱家红[1] 陈雄庭[1] 彭世清[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101 [2]海南大学农学院,海南海口570228

出　　处：《热带作物学报》2017年第2期295-301,共7页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金(No.314115);星火计划重点项目(No.2014GA800003)

摘　　要：利用Genome Walker方法从巴西橡胶树中克隆了Hb SERK1起始密码子上游1 395 bp的5′调控序列,序列分析表明,该序列A/T含量高达66.2%,符合真核生物启动子序列的特征。Hb SERK1启动子序列中含有多个典型的真核生物启动子基本元件,如:TATA-box,CAAT-box等,同时存在其它应答元件,如:CAT-box、02-site、ERE、ARE、TCA-element、GCN4-motif、ACA-motif等。将Hb SERK1启动子进行5′缺失,并构建了5个植物缺失表达载体。利用真空渗透法和农杆菌介导法将植物缺失表达载体转化烟草,对烟草转化植株进行GUS活性定量分析结果表明,除了SP5外,其它缺失表达载体均可以驱动GUS蛋白的表达,说明在Hb SERK1启动子-1 395^-252 bp区域可以驱动GUS的表达,表明Hb SERK1启动子是具有生物活性的启动子。The 5＇ regulatory region of HbSERK1 from Hevea brasiliensis was cloned using GenomeWalker strategy. A/T content in this sequence is up to 66.2%, and meet the characteristic of eukaryotic promoter sequence. TATA box, CAAT box CAT-box, 02-site, ERE, ARE, TCA-element, GCN4-motif, ACA-motif were found in this promoter regioru In order to verify HbSERK1 promoter function, Five deletions plant expression vector were constructed and transformed to the tobacco by Agrobacterium-mediated vacuum infiltration method and Agrobacterium-mediated. GUS activity analysis revealed that the region from -1 395-252 bp nt drived expression of the GUS gene except SP5 deletion （-252 bp）. The results of transient expression and stable expression suggest HbSERK1 promoter possess bioactivity.

关 键 词：巴西橡胶树 HB SERK1启动子 功能鉴定 

分 类 号：S794.1[农业科学—林木遗传育种]