绵羊肺腺瘤病毒缺失型SU蛋白真核表达载体的构建  

Construction of eukaryotic expression vector for SU defective protein of JSRV

作　　者：张月梅[1] 马学恩[2] 么宏强[2] 赵世华[1] 

机构地区：[1]内蒙古农牧业科学院兽医所,内蒙古呼和浩特010031 [2]内蒙古农业大学兽医学院,内蒙古呼和浩特010018

出　　处：《中国兽医学报》2017年第3期420-425,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31101788)

摘　　要：试验证明绵羊透明质酸酶2(hyaluronidase 2,Hyal-2)可以作为绵羊肺腺瘤病毒的受体与绵羊表面蛋白(surface protein,SU)亚基结合。为了进一步研究绵羊Hyal-2与SU蛋白可能结合的区域,运用生物信息学软件预测了SU蛋白的膜外区部分,然后分别构建了一系列缺失型真核表达载体,将缺失型SU蛋白真核表达载体pEGFP-C1-(su1~su5)转染293T细胞,激光共聚焦显微镜观察结果显示:SU1-SU5融合蛋白定位于细胞质,Western blot结果显示在66 000处出现阳性条带,表明成功构建了SU蛋白缺失型真核表达载体。The experimental results showed that Hyal-2 can be used as a receptor for JSRV of sheep and combination with SU protein subunits. In order to further study on the possible binding re- gions of Hyal-2 and SU protein,bioinformatics software was used to predict the ectodomain part of Su protein, respectively, then constructed a series of eukaryotic expression vectors for preparing SU defective proteins. The expression vectors of pEGFP-C1- （sul-su5） were transfected into 293T cells,confocal laser scanning microscopy results shown that SU1-SU5 fusion protein was localized in the cytoplasm,Western blot results displayed a positive band in the 66 000,indicating that eu- karyotic expression vectors of SU defective protein were successfully constructed.

关 键 词：缺失型蛋白 真核表达载体 结合区域 激光共聚焦 

分 类 号：S857.4[农业科学—临床兽医学]