ACTB基因第4外显子遗传变异分析及其与种公牛精液品质的关系  

Genetic variation analysis of exon 4 in ACTB gene and correlation analysis with semen quality in bulls

作　　者：岳续朋[1,2] 贺洪涛 张志刚 戴立胜[2] 赵锐锋 张嘉保[2] 

机构地区：[1]遵义医学院珠海校区,广东珠海519041 [2]吉林大学实验动物中心,吉林长春130062 [3]涿州市动物卫生监督所,河北涿州072750 [4]吉林省长春优质肉牛繁育中心,吉林长春130062

出　　处：《中国兽医学报》2017年第3期551-553,共3页Chinese Journal of Veterinary Science

基　　金：肉牛牦牛产业技术体系资助项目(CARS-38);国家科技支撑计划资助项目(2011BAD19B04)

摘　　要：采用PCR-SSCP技术对45头种公牛的ACTB(β肌动蛋白)基因的第4外显子的单核甘酸多态性进行了检测。结果显示:在ACTB基因第4外显子上存在2种基因型,即AA型和AB型。对该多肽片段克隆测序,结果在ACTB基因第4外显子上存在G378C突变。用最小二乘法分析ACTB基因的第4外显子不同基因型与精液品质的相关性,发现ACTB基因第4外显子AA型精子浓度、鲜精畸形率以及冻精畸形率显著高于AB型(P<0.05),AA型鲜精活力、鲜精活精子比率极显著高于AB型(P<0.01),AA型冻精活精子比率显著低于AB型(P<0.05)。The single nucleotide polymorphism of the ACTB gene in cattle was analyzed by PCR- SSCP. The results showed that two genotypes, AA and AB were detected in exon 4 of ACTB gene. The polymorphism fragments were cloned and sequenced. The sequencing results indicted there was one single nucleotide mutations：G378C in exon 4 of ACTB gene. The genetic correlations were obtained between genotypes of the exon 4 of ACTB gene and semen quality in cattles by the least square analysis. The results indicated that different genotypes of ACTB in exon 4 significantly af- fected semen quality. Bulls with the AA genotype had a significantly higher sperm concentration and a significantly higher percentage of deformity in fresh and frozen semen than those of the AB genotypes （P〈0.05）. Further,bulls with the AA genotypes showed an significantly lower per- centage of living semen （P〈0.01） and a significantly lower percentage of acrosome integrity （P〈 0.05） in fresh than those of the AB genotypes.

关 键 词：牛 ACTB基因 PCR SSCP 单核甘酸多态性(SNP) 

分 类 号：S823[农业科学—畜牧学]