ε-聚赖氨酸在牛胚胎玻璃化冷冻中的保护作用  

Protective effect of ε-Poly-L-lysine on bovine embryo vitrification

作　　者：钟原[1] 高伟[1] 姜昊[1] 张明军[1] 张嘉保[1] 俞先峰[1] 

机构地区：[1]吉林大学动物科学学院,吉林长春130062

出　　处：《中国兽医学报》2017年第3期554-558,共5页Chinese Journal of Veterinary Science

基　　金：现代农业(肉牛牦牛)产业技术体系建设专项资助项目(CARS-38)

摘　　要：通过研究ε-聚赖氨酸(ε-PL)对牛胚胎玻璃化冷冻的影响,探讨ε-PL在牛胚胎玻璃化冷冻中的保护作用,为优化牛胚胎玻璃化冷冻技术提供依据。单独以ε-PL作为冷冻保护剂,其质量浓度为20、35、50g/L,分别进行胚胎冷冻,结果均不能取得成活胚胎,说明ε-PL不能单独作为冷冻保护剂使用。在传统玻璃化冷冻剂中分别添加质量浓度为10、20、30g/L的ε-PL进行胚胎冷冻,结果发现添加ε-PL之后的冷冻保护液具有更好的保护效果,且ε-PL质量浓度为20g/L时效果最好,囊胚解冻孵化率可达42.37%。进一步探索了用该冷冻保护剂,缓冲时间分别为5、7.5、10min进行胚胎冷冻的效果,结果发现缓冲时间为7.5 min取得最高孵化率,孵化率为46.58%。结果表明,ε-PL对牛胚胎玻璃化冷冻具有一定的保护作用,且适当延长缓冲时间可取得更好的冷冻效果。To study the protective effect of ε-PL on bovine embryo vitrification and provide a refer- ence basis for optimizing bovine embryo vitrification technology, three bovine embryo vitrification contrast tests with ε-PL as the only cryoprotectant as 20,35 and 50 g/L concentration did not ac quired live embryo after thawed in these groups. It suggests that single ε-PL could not be used as eryoprotectant. Then we performed a bovine embryo vitrification test with traditional cryopro- tectant adding ε-PL as different concentration 10,20 and 30 g/L. The result showed that 20 g/L ε- PL performs the best hatchability,up to 42. 37%. In condition of adding 20 g/L ε-PL in traditional cryoprotectant, compared with 5 and 10min of action period for vitrification. We observed that 7.5 rain of the highest hatchability,up to 46.58%. These results demonstrate that the ε-PL has protec- tive effect on bovine embryo vitrification, and properly extending action period will be helpful to improving its protective effect.

关 键 词：ε-聚赖氨酸(ε-PL) 牛胚胎 玻璃化冷冻 

分 类 号：S814.8[农业科学—畜牧学]