高压电烧伤对大鼠小肠浆膜表面微循环灌流量的影响及己酮可可碱的干预作用  

Influences of high-voltage electrical burns on microcirculation perfusion on serosal surface of small intestine of rats and the interventional effects of pentoxifylline

作　　者：张庆富[1] 许顺江[1] 梁利民[1] 冯建科[1] 徐彦粉[1] 屠丽红[1] 

机构地区：[1]河北医科大学第一医院烧伤整形外科,河北省烧伤工程技术研究中心,石家庄050031

出　　处：《中华烧伤杂志》2017年第3期166-170,共5页Chinese Journal of Burns

基　　金：基金项目：河北省自然科学基金（C2011206080）;河北省应用基础研究计划重点基础研究项目（14967724D）;河北省省级重大医学科研课题（zd2013080）

摘　　要：目的探讨高压电烧伤对大鼠小肠浆膜表面微循环灌流量的影响以及己酮可可碱（PTX）的干预作用。 方法将180只SD大鼠按随机数字表法分为假伤组、单纯电伤组和治疗组，每组60只。以大鼠左前肢近心端外侧为电流入口，右后肢近心端外侧为电流出口，单纯电伤组和治疗组大鼠用调压器及实验变压器造成入口和出口处各1 cm×1 cm的高压电烧伤创面；假伤组大鼠接相同装置但不通电致假伤。伤后2 min，假伤组和单纯电伤组大鼠腹腔注射生理盐水2 mL，治疗组大鼠腹腔注射50 mg/mL PTX注射液2 mL。伤前15 min及伤后5 min、1 h、2 h、4 h、8 h，每组各取10只大鼠，经心脏采血分离血清，用ELISA法检测血清可溶性血管细胞黏附分子1（sVCAM－1）含量；用布莱德多项投影显微镜系统检测肠系膜微静脉白细胞黏附数；用激光多普勒血流灌注成像仪检测小肠浆膜表面微循环灌流量。对数据行析因设计方差分析和LSD检验。 结果（1）单纯电伤组大鼠伤后5 min、1 h、2 h、4 h、8 h血清sVCAM－1含量分别为（8 502±1 158）、（11 793±3 310）、（9 960±2 146）、（9 708±1 429）、（7 292±1 386）ng/mL，均高于假伤组的（1 897±946）、（1 882±940）、（1 882±938）、（1 888±946）、（1 884±942）ng/mL和治疗组的（6 840±1 558）、（6 742±2 465）、（5 625±2 593）、（2 373±1 463）、（5 187±2 797）ng/mL（P值均小于0.001）。单纯电伤组大鼠伤后各时相点、治疗组大鼠伤后各时相点（除伤后4 h外）血清sVCAM－1含量均高于组内伤前15 min（P值均小于0.001）。（2）单纯电伤组大鼠伤后各时相点肠系膜微静脉白细胞黏附数均多于假伤组和治疗组（P值均小于0.001）。单纯电伤组和治疗组大鼠伤后各时相点肠系膜微静脉白细胞黏附数均多于组内伤前15 min（P值均小于0.001）。（3）单纯电伤组大鼠伤后各时相点小肠浆膜表�ObjectiveTo investigate influences of high-voltage electrical burns on microcirculation perfusion on serosal surface of small intestine of rats and the interventional effects of pentoxifylline （PTX）. MethodsTotally 180 SD rats were divided into sham injury group, simple electrical burn group, and treatment group according to the random number table, with 60 rats in each group. The electrical current was applied to the outside proximal part of left forelimb of rats and exited from the outside proximal part of right hind limb of rats. Rats in simple electrical burn group and treatment group were inflicted with high-voltage electrical burn wounds of 1cm×1cm at current entrances and exits, with the voltage regulator and experimental transformer. Rats in sham injury group were sham injured through connecting the same equipments without electricity. At 2 min post injury, rats in sham injury group and simple electrical burn group were intraperitoneally injected with 2 mL normal saline, and rats in treatment group were injected with 2 mL PTX injection （50 mg/mL）. At 15 min before injury and 5 min, 1 h, 2 h, 4 h, and 8 h post injury, 10 rats in each group were selected to collect blood of heart respectively. Serum were separated from the blood to determine the level of soluble vascular cell adhesion molecule-1（sVCAM-1） with enzyme-linked immunosorbent assay method. The number of adhesional leukocyte in mesenteric venule of rats was determined with Bradford variable projection microscope system. The microcirculation perfusion on serosal surface of small intestine of rats was detected with laser Doppler perfusion imager. Data were processed with analysis of variance of factorial design and LSD test. Results（1） At 5 min, 1 h, 2 h, 4 h, 8 h post injury, the serum content of sVCAM-1 in rats of simple electrical burn group were （8 502±1 158）, （11 793±3 310）, （9 960±2 146）, （9 708±1 429）, （7 292±1 386） ng/mL respectively, higher than that in sham injury group and treatment group [

关 键 词：烧伤 电 白细胞 微循环 己酮可可碱 可溶性血管细胞黏附分子1 

分 类 号：R647[医药卫生—外科学]