邢台地区致鸡卵黄性腹膜炎大肠杆菌血清型鉴定、毒力基因检测及耐药性分析  

Investigation of serotypes, virulence genes and antimicrobial susceptibility of the Escherichia coli isolates causing chicken salpingitis and peritonitis in XingTai area

作　　者：张召兴[1] 李蕴玉[1] 吴同垒[1] 张香斋[1] 张志强[1] 史秋梅[1] 张艳英[1] 贾青辉[1] 李佩国[1] 

机构地区：[1]河北科技师范学院河北省预防兽医学重点实验室,河北秦皇岛066604

出　　处：《中国预防兽医学报》2017年第3期191-195,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：河北省现代农业产业技术体系蛋鸡产业创新团队岗位(HBCT2013090203);河北省研究生创新资助项目(2016~2017);河北省科技支撑项目(12220412D);河北省高等学校科学技术研究项目(2015064)

摘　　要：为分析邢台地区致鸡卵黄性腹膜炎大肠杆菌(E.coli)分离株的血清型、毒力基因和耐药性,本实验采用常规鉴定方法和16S rRNA PCR的方法,从邢台地区不同养鸡场46份患卵黄性腹膜炎的40周龄~45周龄蛋鸡病料组织中分离鉴定出37株E.coli。人工感染1日龄的雏鸡,结果显示32株分离菌为致病性E.coli,采用玻板凝集法检测其血清型分布,结果显示定型的27株致病性E.coli分离株共属于11个血清型,以O_(78)、O_(148)、O_2及O_8为优势血清型。采用PCR方法检测32株致病性E.coli分离株14种毒力基因,其中irp2、fyuA、ompT、Iss^a、iucD^a、iutA、iroN、hlyF、fimC毒力基因检出率最高,分别为100%、96.9%、93.8%、86.7%、84.3%、71.9%、65.7%、56.3%和50%,其余毒力基因检出率较低。耐药性分析结果显示,32株致病性E.coli分离株仅对头孢克肟、左氧氟沙星、阿奇霉素、头孢曲松和氟苯尼考5种药物高度敏感,对其他药物存在不同程度的耐药性。本研究为防治致病性E.coli引起蛋鸡卵黄性腹膜炎提供了实验依据。To investigate the serotype, virulence genes and drug resistance of the E.coli isolates causing chicken salpingitis and peritonitis in Xingtai area, a total of 37 E.coli were isolated from 46 salpingitis and peritonitis samples of 40-45 weeks old chicken in different chicken farms in Xingtai area, and identified by bacteria characteristics and 16S rRNA PCR methods. The results of the artificial infection in 1 days old chickens showed that 32 isolates were pathogenic E.coli; The serotype of 32 isolates were determined by direct agglutination test with specific antisera, 27 pathogenic E.coli isolates belonged to 11 kinds of serotypes, of which O78, O148, O2 and O8 were the predominant serotypes. The positive rates of the virulent genes of irp2, fyuA, ompT, Issa, iucDa, iutA, iroN, hlyF and fimC genes were 100%, 96.9%, 93.8%, 86.7%, 84.3%, 71.9%, 65.7%, 56.3% and 50%, respectively, in the 32 E.coli isolates by PCR method. Assay of drug resistance showed the 32 isolates were highly sensitive to 5 kinds of drugs, such as levofloxacin, azithromycin, ceftriaxone, florfenicol. The sensibility to other drugs was low. These data provide the basis for the prevention and treatment of the E.coli causing chicken salpingitis and peritonitis.

关 键 词：大肠杆菌 卵黄性腹膜炎 血清型 毒力基因 耐药性 

分 类 号：S852.61[农业科学—基础兽医学]